Donkey anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]


Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase] [NBP1-72681] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole more

Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
1 mg/ml

Order Details

Donkey anti-Rat IgG (H L) Secondary Antibody [Alkaline Phosphatase] Summary

Rat IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Alkaline Phosphatase
Alkaline Phosphatase
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:2,000 is suggested for this product.

Reactivity Notes

Reacts with Rat.


Label Molecular Weight: 140,000


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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