| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52663-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48330 | |||
| Azide and BSA Free | NBP3-11580 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44393 | |||
| Azide and BSA Free | NBP2-34680-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25264 | |||
| Azide and BSA Free | NBP2-25264SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80706 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80710 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47936-0.1mg | |||
| IHC-Prediluted | NBP2-48021 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80709 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24858SS | |||
![Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Western-Blot-NBP2-67608-img0001.jpg "Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human lung tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0005.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human tonsil tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human small intestine tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0003.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human prostate carcinoma tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0004.jpg)
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0003.jpg "Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0001.jpg)
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0002.jpg)
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Western-Blot-NBP2-44340-img0005.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Tonsil stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0003.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0004.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0002.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0001.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (M2-7C10+M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-+-M2-9E3-Immunohistochemistry-Paraffin-NBP2-34245-img0001.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) [NBP2-34245] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (M2-7C10+M2-9E3). Note cytoplasmic staining of cells.")
![Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-73N8F2-Western-Blot-NBP2-25249-img0005.jpg "Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Mouse testis stained with IL-7R antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-73N8F2-Immunohistochemistry-Paraffin-NBP2-25249-img0004.jpg)
![Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Analysis of mouse thymus tissue using EBI3 antibody at a concentration of 1 ug/ml.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Western-Blot-NBP2-03940-img0007.jpg "Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Analysis of mouse thymus tissue using EBI3 antibody at a concentration of 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Figure A: Intracellular analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Flow-Cytometry-NBP2-03940-img0005.jpg)
![Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Western blot testing of mouse EBI3 monoclonal antibody at A) 0.1 ug/ml on recombinant protein and B) 3 ug/ml on recombinant mouse IL-35 protein. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Western-Blot-NBP2-03940-img0006.jpg)
![Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (42N1G4) [NBP2-27356] - Analysis using EBI3 antibody. Human EBI3 recombinant protein (A) and human IL-35 recombinant protein (B) probed with EBI3 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-42N1G4-Western-Blot-NBP2-27356-img0004.jpg "Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (42N1G4) [NBP2-27356] - Analysis using EBI3 antibody. Human EBI3 recombinant protein (A) and human IL-35 recombinant protein (B) probed with EBI3 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (42N1G4) [NBP2-27356] - Intracellular analysis of EBI3 in transfected cells using EBI3 antibody at 0.2 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-42N1G4-Flow-Cytometry-NBP2-27356-img0003.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (LAMP3/968) [NBP2-44810] - Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Antibody (LAMP3/968)](http://images.novusbio.com/fullsize/CD63-Antibody-LAMP3-968-Immunohistochemistry-Paraffin-NBP2-44810-img0006.jpg "Immunohistochemistry-Paraffin: CD63 Antibody (LAMP3/968) [NBP2-44810] - Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Antibody (LAMP3/968)")
![Flow Cytometry: CD63 Antibody (LAMP3/968) [NBP2-44810] - Flow cytometry of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or stained with CF555-labeled CD63 monoclonal antibody (LAMP3/968) (orange)](http://images.novusbio.com/fullsize/CD63-Antibody-LAMP3-968-Flow-Cytometry-NBP2-44810-img0009.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (LAMP3/968) [NBP2-44810] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD63 Antibody (LAMP3/968)](http://images.novusbio.com/fullsize/CD63-Antibody-LAMP3-968-Immunohistochemistry-Paraffin-NBP2-44810-img0005.jpg)
![Flow Cytometry: CD63 Antibody (LAMP3/968) [NBP2-44810] - Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Antibody (LAMP3/968) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD63-Antibody-LAMP3-968-Flow-Cytometry-NBP2-44810-img0007.jpg)
![Flow Cytometry: CD63 Antibody (LAMP3/968) [NBP2-44810] - Flow cytometry of MCF-7 cells unstained (gray) or stained with CF555-labeled CD63 monoclonal antibody (LAMP3/968) (orange).](http://images.novusbio.com/fullsize/CD63-Antibody-LAMP3-968-Flow-Cytometry-NBP2-44810-img0008.jpg)
![Flow Cytometry: CD63 Antibody (LAMP3/968) [NBP2-44810] - An intracellular stain was performed on A431 cells with CD63 [LAMP3/968] Antibody NBP2-47936B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/nbp2-44810_mouse-monoclonal-cd63-antibody-lamp3-968-17720241044619.jpg)
![Western Blot: c-Maf Antibody [NB600-267] - Nuclear extract (6 ug) from HeLa cells transfected with empty vector or expression constructs for mouse MafA, mouse MafB or mouse cMaf. Antibody used at 0.4 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NB600-267-img0005.jpg "Western Blot: c-Maf Antibody [NB600-267] - Nuclear extract (6 ug) from HeLa cells transfected with empty vector or expression constructs for mouse MafA, mouse MafB or mouse cMaf. Antibody used at 0.4 ug/ml.")
![Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P10D3-Western-Blot-NBP2-03943-img0003.jpg "Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943] - Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 2 ug/10^6 cells.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P10D3-Flow-Cytometry-NBP2-03943-img0002.jpg)
![Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP2-24858-img0003.jpg "Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.")
![Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IL-33-Antibody-Simple-Western-NBP2-24858-img0002.jpg)