| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38297-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88057-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77030-0.025mg | |||
![Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Western-Blot-NBP1-52654-img0006.jpg "Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: RFX1 Antibody [NBP1-52654] - Section stained with anti-RFX1 antibody. RFX1 belongs to Cluster 4. Spermatids were stained in brown. These distribution patterns were mostly consisted to that of trancripts. (Abbreviation: SPT=spermatid).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26753906/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-NBP1-52654-img0008.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0004.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunoprecipitation-NBP1-52654-img0007.jpg)
![Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Western-Blot-NBP2-01756-img0002.jpg "Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.")
![Immunocytochemistry/Immunofluorescence: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RFXANK.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunocytochemistry-Immunofluorescence-NBP2-01756-img0010.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0009.jpg)
![Flow Cytometry: RFXANK Antibody (OTI3E7) [NBP2-01756] - Analysis of Jurkat cells, using anti-RFXANK antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Flow-Cytometry-NBP2-01756-img0001.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0003.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0004.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0005.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0006.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human Kidney tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0007.jpg)
![Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Human pancreas tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Immunohistochemistry-Paraffin-NBP2-01756-img0008.jpg)
![Western Blot: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Western-Blot-NBP2-73870-img0001.jpg "Western Blot: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.")
![Immunocytochemistry/Immunofluorescence: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RFXANK.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73870-img0004.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0011.jpg)
![Flow Cytometry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Analysis of Jurkat cells, using anti-RFXANK antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73870-img0003.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0005.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0006.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0007.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0008.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Human Kidney tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0009.jpg)
![Immunohistochemistry: RFXANK Antibody (OTI3E7) - Azide and BSA Free [NBP2-73870] - Staining of paraffin-embedded Human pancreas tissue using anti-RFXANK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RFXANK-Antibody-OTI3E7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73870-img0010.jpg)
![Western Blot: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Western blot analysis of HLA DR/DP on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Western-Blot-NBP2-67610-img0013.jpg "Western Blot: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Western blot analysis of HLA DR/DP on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant")
![Immunocytochemistry/Immunofluorescence: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Staining HLA-DR in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunocytochemistry-Immunofluorescence-NBP2-67610-img0009.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HLA DR/DP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-66, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0012.jpg)
![Flow Cytometry: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of Jurkat cells with HLA-DR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Flow-Cytometry-NBP2-67610-img0001.jpg)
![Western Blot: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of HLA-DR on Daudi cells lysates using anti-HLA-DR antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Western-Blot-NBP2-67610-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Staining HLA-DR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunocytochemistry-Immunofluorescence-NBP2-67610-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of paraffin-embedded human kidney tissue using anti-HLA-DR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of paraffin-embedded human liver tissue using anti-HLA-DR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of paraffin-embedded human spleen tissue using anti-HLA-DR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of paraffin-embedded human tonsil tissue using anti-HLA-DR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Analysis of paraffin-embedded mouse kidney tissue using anti-HLA-DR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0007.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HLA DR/DP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-66, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0010.jpg)
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (SC06-78) [NBP2-67610] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HLA DR/DP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-66, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/HLA-DR-DP-Antibody-SC06-78-Immunohistochemistry-Paraffin-NBP2-67610-img0011.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0003.jpg "Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Flow Cytometry: c-jun Antibody (5B1) [NBP2-37576] - Analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Flow-Cytometry-NBP2-37576-img0005.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human intima cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0006.jpg)
![ELISA: c-jun Antibody (5B1) [NBP2-37576] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-ELISA-NBP2-37576-img0001.jpg)
![Western Blot: Viperin Antibody [NBP2-03971] - Analysis of viperin using viperin polyclonal antibody. Human brain lysate in the absence (1) and presence (2) of immunizing peptide, mouse brain (3) and rat brain (4) lysate probed with viperin antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Viperin-Antibody-Western-Blot-NBP2-03971-img0003.jpg "Western Blot: Viperin Antibody [NBP2-03971] - Analysis of viperin using viperin polyclonal antibody. Human brain lysate in the absence (1) and presence (2) of immunizing peptide, mouse brain (3) and rat brain (4) lysate probed with viperin antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Viperin Antibody [NBP2-03971] - Analysis of viperin using viperin polyclonal antibody. Human rectum tissue probed with viperin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Viperin-Antibody-Immunohistochemistry-Paraffin-NBP2-03971-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cell line HaCaT shows localization to nucleus, plasma membrane & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38297-img0003.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cell line HaCaT shows localization to nucleus, plasma membrane & cell junctions. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunohistochemistry-Paraffin-NBP2-38297-img0007.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human skin shows moderate to strong membranous positivity in keratinocytes.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunohistochemistry-Paraffin-NBP2-38297-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human endometrium shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunohistochemistry-Paraffin-NBP2-38297-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cervix, uterine shows moderate to strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunohistochemistry-Paraffin-NBP2-38297-img0006.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0007.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0002.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP1-88057] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88057-img0012.jpg "Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP1-88057] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Plastin L Antibody [NBP1-88057] -Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88057_rabbit-polyclonal-plastin-l-antibody-immunohistochemistry-paraffin-2282024113244..jpg)
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0012.jpg "Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0006.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0011.jpg)
![Immunohistochemistry: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunohistochemistry Validation of SCF/c-kit Ligand in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF/c-kit Ligand antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-2711202420441113.jpg)
![Immunocytochemistry/ Immunofluorescence: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunofluorescence Validation of SCF/c-kit Ligand in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF/c-kit Ligand with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-271120241928812.jpg)
![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0006.jpg "Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of various cell lines, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0005.jpg)
![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Rat brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632] - Confocal imaging of paraffin-embedded Human kidney using CHD3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-52202511411435.jpg)
![Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632] - Confocal imaging of paraffin-embedded Mouse brain using CHD3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-5220251265342.jpg)
![Immunohistochemistry: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry analysis of CHD3 in paraffin-embedded rat spleen tissue using CHD3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-522025125490.jpg)
![Immunohistochemistry: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry analysis of CHD3 in paraffin-embedded human tonsil tissue using CHD3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-5220251265339.jpg)
![Immunohistochemistry: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry analysis of CHD3 in paraffin-embedded human cervix cancer tissue using CHD3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-522025125557.jpg)
![Immunohistochemistry: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry analysis of CHD3 in paraffin-embedded mouse brain tissue using CHD3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-52202511361221.jpg)
![Immunohistochemistry: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry analysis of CHD3 in paraffin-embedded mouse kidney tissue using CHD3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16632_rabbit-chd3-mab-8z6l6-52202511411470.jpg)