| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33518-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24858SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24558-25ul | |||
| Azide and BSA Free | NBP3-43843 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-63062-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-2005-0.002g | |||
| HRP | NB600-850-0.1mg | |||
![Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Western-Blot-NBP2-32500-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IFIT3 Antibody [NBP2-32500] - Staining of human cell line A-431 shows localization to cytosol & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32500-img0016.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0026.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0020.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0024.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0025.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lysozyme Antibody [NBP2-33518] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP2-33518-img0005.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human stomach shows moderate extracellular positivity in glandular cells.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-NBP2-33518-img0016.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human kidney shows strong extracellular positivity in cells in tubules.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0013.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human lymph node shows moderate extracellular positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0014.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0015.jpg)
![Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-278202415212450.jpg "Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-298202414333619.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-29202410484842.jpg)
![Flow Cytometry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Flow cytometric analysis of NIH/3T3 cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32356, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-392024884678.jpg)
![Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0005.jpg "Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0001.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0003.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0004.jpg)
![Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-278202416331261.jpg "Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29820241492613.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunocytochemistry analysis of HeLa cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29202410334066.jpg)
![Flow Cytometry: FXR1 Antibody (JE40-57) [NBP3-32354] - Flow cytometric analysis of HeLa cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-392024884674.jpg)
![Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP2-24858-img0003.jpg "Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.")
![Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IL-33-Antibody-Simple-Western-NBP2-24858-img0002.jpg)
![CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-BGN-0696-5B1-CyTOF-ready-NB100-63062-img0001.jpg "CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.")
![Western Blot: NOXO1 Antibody [NBP1-77899] - NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown).](http://images.novusbio.com/fullsize/NOXO1-Antibody-Western-Blot-NBP1-77899-img0001.jpg "Western Blot: NOXO1 Antibody [NBP1-77899] - NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown).")
![Immunohistochemistry: NOXO1 Antibody [NBP1-77899] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of the lamina propia in human colon epithelium and macrophages at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/NOXO1-Antibody-Immunohistochemistry-NBP1-77899-img0003.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0003.jpg "Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: COL4A3BP Antibody [NBP1-31118] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31118-img0002.jpg)
![Immunohistochemistry-Paraffin: COL4A3BP Antibody [NBP1-31118] - BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-31118-img0004.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - A: Hep G2 B: MOLT4 C: Raji 12% SDS PAGE; antibody diluted at 1:500](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0001.jpg)
![Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-278202415295525.jpg "Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32355) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-29820241435234.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-2920241192383.jpg)
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0010.jpg "Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0009.jpg)
![ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-ELISA-NBP1-77790-img0011.jpg)