| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24858SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24558-25ul | |||
| Azide and BSA Free | NBP3-43843 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85781-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-23442-25ug | |||
| BSA Free | NBP3-23442-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76740SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45086 | |||
| Azide and BSA Free | NBP2-34660-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75060 | |||
| Pre-adsorbed | NBP1-75062 | |||
| Pre-adsorbed | NBP1-75063 | |||
| Pre-adsorbed | NBP1-75064 | |||
| Pre-adsorbed | NBP1-75937 | |||
| Pre-adsorbed | NBP1-75060-0.5mg | |||
| Pre-adsorbed | NBP1-75060JF549 | |||
| Pre-adsorbed | NBP1-75060JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71112 | |||
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Western-Blot-NBP2-75441-img0006.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0005.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0003.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0007.jpg)
![Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP2-24858-img0003.jpg "Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.")
![Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IL-33-Antibody-Simple-Western-NBP2-24858-img0002.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining in human tonsil and cerebral cortex tissues using anti-LAG3 antibody. Corresponding LAG3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining in human tonsil and cerebral cortex tissues using anti-LAG3 antibody. Corresponding LAG3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0006.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0005.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0007.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IL-21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76740-img0008.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0005.jpg)
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740_rabbit-polyclonal-il-21-antibody-271120241937752.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Western-Blot-NBP2-27551-img0002.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Tonsil (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0006.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of human skin Epidermis shows detection on the apical side of the basal cell layer.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Testis (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0005.jpg)
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (KLC709).](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Immunohistochemistry-Paraffin-NBP2-34259-img0007.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (KLC709).")
![Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of H. Plasma lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Simple-Western-NBP2-34259-img0003.jpg)
![Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Electropherogram image of the corresponding Simple Western lane. Kappa Light Chain antibody was used at 10 ug/ml dilution of H. Plasma lysates(s) respectively.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Simple-Western-NBP2-34259-img0004.jpg)
![Protein Array: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Kappa Light Chain Antibody (KLC709).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Protein-Array-NBP2-34259-img0008.jpg)
![Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Western-Blot-NBP3-05777-img0001.jpg "Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).")
![Immunohistochemistry-Paraffin: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil tissue stained with LAG-3 (human) recombinant mAb (L4-PL33) at 6ug/ml.](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Immunohistochemistry-Paraffin-NBP3-05777-img0002.jpg)
![Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Western-Blot-NBP2-45581-img0004.jpg "Western Blot: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3.")
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Immunohistochemistry-NBP2-45581-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (1:100).](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Flow-Cytometry-NBP2-45581-img0005.jpg)
![Immunohistochemistry: LAG-3 Antibody (OTI8F6) [NBP2-45581] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/LAG-3-Antibody-OTI8F6-Immunohistochemistry-NBP2-45581-img0001.jpg)