| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25241SS | |||
| Azide and BSA Free | NBP2-80965 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27092 | |||
| BSA Free | NBP2-27091SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76465-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76739-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0002.jpg "Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]")
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0001.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Sections of nasal biopsies were incubated with antibodies against TLR9 (C) visualized by 3, 3-diaminobenzidine (brown). In control slides (F), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0098239) licensed under a CC-BY license. TLR7 Antibody [NB100-56682]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0003.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz. TLR7 Antibody [NBP2-24767]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0004.jpg)
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary. TLR7 Antibody [NB100-56588]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0005.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Western-Blot-NBP2-25241-img0001.jpg "Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.")
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Immunocytochemistry-Immunofluorescence-NBP2-25241-img0003.jpg)
![Western Blot: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis using IL-23 receptor antibody. Lysate from (A) human HCT116, (B) Jurkat and C) mouse NIH 3T3 cells probed with IL-23 receptor antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Western-Blot-NBP2-27091-img0004.jpg "Western Blot: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis using IL-23 receptor antibody. Lysate from (A) human HCT116, (B) Jurkat and C) mouse NIH 3T3 cells probed with IL-23 receptor antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: IL-23 R Antibody (15N6C6) [NBP2-27091] - Human Appendix FFPE tissue section stained with IL-23 R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Immunohistochemistry-Paraffin-NBP2-27091-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis of IL-23 receptor in human colon tissue using IL-23 receptor antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Immunohistochemistry-Paraffin-NBP2-27091-img0003.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0005.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Paraffin-embedded human melanoma. pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0004.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249342769.jpg "Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249432836.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249422675.jpg)
![Immunocytochemistry/Immunofluorescence: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-212024940844.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249425497.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-212024941481.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody (HL1418) - Azide and BSA Free [NBP3-25463] - FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FCER1G stained by FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-2120249393517.jpg)
![Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (NBP3-25463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25463_rabbit-fcer1g-mab-hl1418-azide-and-bsa-free-western-blot-2611202518263616.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202419463463.gif "<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Western Blot Validation of MyD88 in human cell lines. Loading: 15 ug of lysates per lane. Antibodies: NBP1-76465 (2 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0010.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - Animal Species ReactivityLoading: Lysates/proteins at 15 ug per lane.Antibodies: NBP1-76925 (2 ug/mL) or NBP1-76465 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MyD88 Antibody - BSA Free [NBP1-76465] - Validation of MyD88 In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202420392736.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunocytochemistry-immunofluorescence-22102025124527.gif)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025124757.jpg)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Mouse Heart. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025125425.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-OTI1F12-Immunocytochemistry-Immunofluorescence-NBP2-46125-img0005.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0002.jpg)
![Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-NBP1-76739-img0006.jpg "Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)). White bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76739-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in Western Blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0008.jpg)
![Western Blot: IL-21R Antibody [NBP1-76739] - Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 ug /ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-21R Antibody [NBP1-76739] - Rat lung with IL-21 receptor antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-Paraffin-NBP1-76739-img0001.jpg)
![Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - Treatment of avian xenografts with IL-21 upregulates Blimp-1 expression. (A) The three-dimensional growth of BxPC-3 cells transplanted on the CAM of fertilized chicken eggs & resected tumors (three replicates) from vehicle control & IL-21 were photographed at day 17 of embryonic development. Upper row for vehicle control; lower row for IL-21-treated (150 ng/mL) tumors. (B) HE & IL-21R (brown) IHC staining of vehicle control & IL-21-treated (150 ng/mL) resected xenograft tumors. (C) Comparison & quantification of Blimp-1 (nuclei: red) expression of vehicle control & IL-21-treated (150 ng/mL) xenograft tumors. **** P Green: IL-21 Receptor Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-271120242011556.gif)
![Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-2711202419241320.jpg)
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)