| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43670-100ul | |||
| BSA Free | NBP3-43670-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26866-100ul | |||
| BSA Free | NBP3-26866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26867-100ul | |||
| BSA Free | NBP3-26867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12059-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26373-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47865-0.1mg | |||
| IHC-Prediluted | NBP2-48365 | |||
![Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RP105-Antibody-Western-Blot-NBP2-20204-img0001.jpg "Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-20204] - Mahlarvu xenograft, using RP105 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RP105-Antibody-Immunohistochemistry-Paraffin-NBP2-20204-img0002.jpg)
![Flow Cytometry: RP105/CD180 Antibody [NBP2-20204] - Flow cytometry on Jurkat cells, staining with RP105 antibody at 1:100 dilution (blue) or rabbit IgG (black).](http://images.novusbio.com/fullsize/RP105-Antibody-Flow-Cytometry-NBP2-20204-img0003.jpg)
![Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Western-Blot-NBP3-12916-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Rat lymph node. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse spleen. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0002.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse thymus gland. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0003.jpg)
![Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Western-Blot-NBP1-51958-img0003.jpg "Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human tonsil. Antbody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0004.jpg)
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: HVCN1 Antibody [NBP1-06585] - HVCN1 expression in human peripheral blood naive (CD27-) B cells and memory (CD27+) B cells](http://images.novusbio.com/fullsize/HVCN1-Antibody-Western-Blot-NBP1-06585-img0005.jpg "Western Blot: HVCN1 Antibody [NBP1-06585] - HVCN1 expression in human peripheral blood naive (CD27-) B cells and memory (CD27+) B cells")
![Immunohistochemistry-Paraffin: HVCN1 Antibody [NBP1-06585] - Immunohistochemistry of paraffin-embedded tonsil sections stained with anti-HVCN1](http://images.novusbio.com/fullsize/HVCN1-Antibody-Immunohistochemistry-Paraffin-NBP1-06585-img0001.jpg)
![Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2682024158261.jpg "Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds")
![Western Blot: CD19 Antibody (PSH03-94) [NBP3-32130] - Western blot analysis of CD19 on mouse spleen tissue lysates with Rabbit anti-CD19 antibody (NBP3-32130) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32130) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-278202415212455.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD19 with Rabbit anti-CD19 antibody (NBP3-32130) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32130, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-29820241420976.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-CD19 antibody (NBP3-32130) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32130) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2982024149261.jpg)
![Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD53-Antibody-HD77-Chimeric-Western-Blot-NBP3-12059-img0001.jpg "Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12059 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-74202522265216.png)
![Flow Cytometry: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12059 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-7420252214126.png)
![Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-262202414471581.jpg)
![Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-2722024856312.jpg)
![Western Blot: BOB1 Antibody (4A5) [NBP3-26373] - Positive Western Blot detected in: Raji whole cell lysate. All lanes: BOB1 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-2822024115258.jpg)
![Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) [NBP2-44619] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (CR1/802).](http://images.novusbio.com/fullsize/CD35-Antibody-CR1-802-Immunohistochemistry-Paraffin-NBP2-44619-img0002.jpg "Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) [NBP2-44619] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Antibody (CR1/802).")
![Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) [NBP2-44619] - Human Tonsil stained with CD35 Monoclonal Antibody (CR1/802).](http://images.novusbio.com/fullsize/CD35-Antibody-CR1-802-Immunohistochemistry-Paraffin-NBP2-44619-img0001.jpg)