| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90207-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43792 | |||
| BSA Free | NBP2-46631-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85581-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88204-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43793 | |||
| BSA Free | NBP2-46632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52501-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96907-0.025ml | |||
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0011.jpg "Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human spleen shows cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Western-Blot-NBP2-46631-img0007.jpg "Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2827) [NBP2-46631] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunocytochemistry-Immunofluorescence-NBP2-46631-img0008.jpg)
![Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse striatum shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunohistochemistry-NBP2-46631-img0005.jpg)
![Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Western-Blot-NBP2-46631-img0006.jpg)
![Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat striatum shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunohistochemistry-NBP2-46631-img0001.jpg)
![Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunohistochemistry-NBP2-46631-img0002.jpg)
![Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunohistochemistry-NBP2-46631-img0003.jpg)
![Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2827-Immunohistochemistry-NBP2-46631-img0004.jpg)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human testis shows moderate positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ULBP-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31866-img0014.jpg "Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human testis shows moderate positivity in a subset of cells in seminiferous ducts.")
![Immunocytochemistry/Immunofluorescence: ULBP-3 Antibody [NBP2-31866] - Staining ULBP-3 in methanol-fixed, Jurkat cells using anti-ULBP-3 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ULBP-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31866-img0013.jpg)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-313202511383..png)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-3132025113854..png)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-313202511406..png)
![Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining in human liver and endometrium tissues using NBP1-85581 antibody. Corresponding ASGR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining in human liver and endometrium tissues using NBP1-85581 antibody. Corresponding ASGR1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium, kidney, liver and tonsil using Anti-ASGR1 antibody NBP1-85581 (A) shows similar protein distribution across tissues to independent antibody NBP1-85580 (B).](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0018.jpg)
![Western Blot: ASGPR1 Antibody [NBP1-85581] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Western-Blot-NBP1-85581-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ASGPR1 Antibody [NBP1-85581] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85581-img0008.jpg)
![Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0017.jpg)
![Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0019.jpg)
![Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0020.jpg)
![Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human tonsil shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85581-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88204-img0007.jpg "Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0008.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0014.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0011.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0012.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0013.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88204_rabbit-polyclonal-calpain-2-antibody-652025162443.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0009.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0014.jpg)
![Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0008.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0001.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse hippocampal formation shows strong immunoreactivity in myelinated fibers in the dentate gyrus.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0002.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat basal forebrain shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0004.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse cerebellum shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (2829) [NBP2-46632] - Staining of human cerebral cortex shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-Paraffin-NBP2-46632-img0003.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human cerebellum shows strong positivity in myelinated neural fiebrs.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0012.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0013.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0012.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunofluorescence-NBP2-52501-img0011.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0009.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0002.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunocytochemistry-Immunofluorescence-NBP2-52501-img0006.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0008.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0007.jpg)
![ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-ELISA-NBP2-52501-img0004.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0003.jpg "Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Flow Cytometry: c-jun Antibody (5B1) [NBP2-37576] - Analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Flow-Cytometry-NBP2-37576-img0005.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human intima cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0006.jpg)
![ELISA: c-jun Antibody (5B1) [NBP2-37576] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-ELISA-NBP2-37576-img0001.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NB110-96907-img0008.jpg "Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody [NB110-96907] - Analysis using Rabbit Anti-p38 Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96907-img0007.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - CtsK & NFATc1 expression levels were decreased during osteoclastogenesis by PSTP-3,5-Me treatment. (A) mRNA expression levels of osteoclast-specific markers were determined by RT-PCR in 0 or 6 µM PSTP-3,5-Me-treated cells. *p < 0.05, & **p < 0.01 indicate the statistically significant difference between non-PSTP-3,5-Me-treated groups (0 µM) & PSTP-3,5-Me-treated groups (6 µM) on each day. Mean ± standard error. (B) Western blotting was performed to determine phosphorylation of NF-kB, p-38, ERK1/2, & JNK. Cells were pre-treated with 6 µM PSTP-3,5-Me or vehicle (Ctrl) for 2 h, & then treated with RANKL for the indicated times. beta -Actin expression level was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31540026), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96907_rabbit-polyclonal-p38-alpha-antibody-31020241534327.jpg)