| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35131-1mg | |||
| Carrier-Free | NBP2-35131-100ug | |||
| Carrier-Free | NBP2-35131-500ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28160 | |||
| FITC | NBP1-28159 | |||
| PE | NBP1-28161 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34896-1mg | |||
| Carrier-Free | NBP2-34896-100ug | |||
| Carrier-Free | NBP2-34896-500ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75011 | |||
| Biotin | NBP1-75012 | |||
| DyLight 594 | NBP1-75888 | |||
| FITC | NBP1-75013 | |||
| HRP | NBP1-75014 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00356-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12630-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
![SDS-Page: Mouse IL-4 Protein [NBP2-35131]](http://images.novusbio.com/fullsize/Mouse-IL-4-Protein-SDS-Page-NBP2-35131-img0001.jpg "SDS-Page: Mouse IL-4 Protein [NBP2-35131]")
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0009.jpg "Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.")
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the Biotin conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0006.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the FITC conjugate of NBP1-28158. Staining of less or equal to ug/10^6 BALB/c spleen cells with Rat Anti-Mouse MHC II-FITC, following which small lymphocytes were gated and analyzed on a flow cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the PE conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0008.jpg)
![SDS-Page: Human IL-4 Protein [NBP2-34896]](http://images.novusbio.com/fullsize/Human-IL-4-Protein-SDS-Page-NBP2-34896-img0001.jpg "SDS-Page: Human IL-4 Protein [NBP2-34896]")
![Immunohistochemistry: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Staining of a human tonsil cross section](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-CC1-Immunohistochemistry-NB100-693-img0004.jpg "Immunohistochemistry: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Staining of a human tonsil cross section")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Formalin fixed paraffin embedded human tonsil stained with Mast Cell Chymase antibody.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-CC1-Immunohistochemistry-Paraffin-NB100-693-img0002.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - NBP3-00356 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:34 kDa](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520251154564.png "Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - NBP3-00356 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:34 kDa")
![Immunoprecipitation: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - PSME3 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg Jurkat Whole Cell Lysate 4 uL NBP3-00356 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00356, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 33 kDa](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520251220553.png)
![Immunocytochemistry/ Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP3-00356] - Immunofluorescence staining of PA28 Activator gamma Subunit/PSME3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00356 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp3-00356_rabbit-polyclonal-pa28-activator-gamma-subunit-psme3-antibody-23520252135126.png)
![Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).](http://images.novusbio.com/fullsize/p_2392_1.jpg "Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).")
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0003.jpg "Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.")
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0001.jpg)
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0002.jpg)
![Western Blot: DNAJC2 Antibody [NBP3-12630] - Lane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:71 kDa](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Western-Blot-NBP3-12630-img0001.jpg "Western Blot: DNAJC2 Antibody [NBP3-12630] - Lane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:71 kDa")
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP3-12630] - Staining of human DNAJC2 in human placenta with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP3-12630-img0003.jpg)
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP3-12630] - Staining of human DNAJC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP3-12630-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32002_rabbit-adenosine-a1r-mab-je31-35-298202413585567.jpg "Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Western blot analysis of Adenosine A1R on different lysates with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: U-87 MG cell lysate (15 ug/Lane) Lane 4: SH-SY5Y cell lysate (15 ug/Lane) Lane 5: HeLa cell lysate (15 ug/Lane) Lane 6: Raji cell lysate (15 ug/Lane) Lane 7: NIH/3T3 cell lysate (15 ug/Lane) Lane 8: Human brain tissue lysate (30 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Human cerebellum tissue lysate (30 ug/Lane) Lane 12: Rat cerebellum tissue lysate (30 ug/Lane) Predicted band size: 36.5 kDa Observed band size: 36.5/40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32002) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32002_rabbit-adenosine-a1r-mab-je31-35-392024845353.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)