| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27166-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27165-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97237-0.5mg | |||
| FITC | NBP1-97216-0.5mg | |||
| HRP | NBP1-97250-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-32470-25ul | |||
![Immunohistochemistry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of paraffin-embedded bladder cancer tissues using Siglec-15 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-10420249181910.jpg%3Fv=1666705394 "Immunohistochemistry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of paraffin-embedded bladder cancer tissues using Siglec-15 mouse mAb with DAB staining.")
![Immunocytochemistry/Immunofluorescence: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Hela cells using Siglec-15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920215.jpg%3Fv=1666705394)
![ELISA: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024921150.jpg%3Fv=1666705394)
![Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mouse mAb against PC-2 (1), LNCap (2), HEK293 (3), PC-3 (4), DU145 (5), COS-7 (6), and HEK293-6e (7) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024916213.jpg%3Fv=1666705394)
![Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of THP-1 cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920226.jpg%3Fv=1666705394)
![Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mAb against human Siglec-15 (AA: Extra(20-263)) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920214.jpg%3Fv=1666705394)
![Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Jurkat cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024915820.jpg%3Fv=1666705394)
![ELISA: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-10420249141930.jpg%3Fv=1666705390 "ELISA: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![Western Blot: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis using Siglec-15 mAb against human Siglec-15 (AA: Extra(20-263)) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-10420249141913.jpg%3Fv=1666705390)
![Flow Cytometry: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis of Jurkat cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-1042024915833.jpg%3Fv=1666705390)
![Flow Cytometry: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis of THP-1 cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-1042024920222.jpg%3Fv=1666705390)
![Immunohistochemistry: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis of paraffin-embedded cervical cancer tissues using Siglec-15 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-10420249141946.jpg%3Fv=1666705390)
![Immunocytochemistry/Immunofluorescence: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis of Hela cells using Siglec-15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-1042024920210.jpg%3Fv=1666705390)
![Western Blot: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis using Siglec-15 mouse mAb against PC-2 (1), LNCap (2), HEK293 (3), PC-3 (4), DU145 (5), COS-7 (6), and HEK293-6e (7) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27165_mouse-siglec-15-mab-3f7f3-bsa-free-1042024920236.jpg%3Fv=1666705390)
![Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - Antigen: Dog IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Dog IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgG-Fc-Secondary-Antibody-Dot-Blot-NBP1-72773-img0001.jpg "Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - Antigen: Dog IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Dog IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.")
![ELISA: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - ELISA results of purified Rabbit anti-Canine IgG Fc Secondary antibody tested against purified Canine IgG F(c). Each well was coated in duplicate with 1.0 ug of Canine IgG F(c) (red line), Canine IgG (green line) .The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Gt Anti-Rabbit HRP and substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgG-Fc-Secondary-Antibody-ELISA-NBP1-72773-img0004.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Load: 50 ng per lane. Primary antibody: NBP2-60656 incubated overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NBP2-60656-img0009.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Load: 50 ng per lane. Primary antibody: NBP2-60656 incubated overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60656-img0016.jpg)
![Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP2-60656-img0010.jpg)
![Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Donkey anti-Goat IgG Antibody Texas Red Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the Texas Red version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP2-60656-img0011.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - ELISA results of purified Donkey anti-Goat IgG antibody Biotin conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 ug of Goat IgG. The starting dilution of antibody was 5 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer, and TMB substrate.. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-ELISA-NBP2-60656-img0013.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60656-img0017.jpg)
![Western Blot: NS3 Antibody [NBP2-42893] - Analysis of A. 30 ug BHK-21 whole cell extract B. 30 ug whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 ug whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 ug whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 ug whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5 % SDS-PAGE NS3 (Dengue virus) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/NS3-Antibody-Western-Blot-NBP2-42893-img0003.jpg "Western Blot: NS3 Antibody [NBP2-42893] - Analysis of A. 30 ug BHK-21 whole cell extract B. 30 ug whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 ug whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 ug whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 ug whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5 % SDS-PAGE NS3 (Dengue virus) antibody dilution: 1:2000")
![Immunocytochemistry/Immunofluorescence: NS3 Antibody [NBP2-42893] - Analysis of Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/NS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42893-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NS3 Antibody [NBP2-42893] - Analysis of Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/NS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42893-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NS3 Antibody [NBP2-42893] - Analysis of Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/NS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42893-img0006.jpg)
![Western Blot: Dengue Virus NS3 Antibody [NBP2-42893] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue Virus NS3 antibody (NBP2-42893) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42893_rabbit-polyclonal-dengue-virus-ns3-antibody-91020242011447.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0012.jpg "Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.")
![Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-NBP1-32505-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0011.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242014696.jpg)
![Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] - Amphiphysin/AMPH antibody [N1N2], N-term detects Amphiphysin/AMPH protein by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Amphiphysin/AMPH stained by Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:500.</br>Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-9102024204124.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242001078.jpg)
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60672-img0011.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc). Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60672-img0007.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0012.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat anti-Rat IgM Heavy Chain Secondary Antibody-Fluorophore-linked immunosorbent assay-NBP2-60672-img0014.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0013.jpg)
![Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP2-53088-img0001.jpg "Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53088-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP2-53088-img0002.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Western Blot: TRAM/TICAM2 Antibody [NBP2-24638] - Lysate from human lung in the 1) absence, 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung probed with TRAM antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Western-Blot-NBP2-24638-img0004.jpg "Western Blot: TRAM/TICAM2 Antibody [NBP2-24638] - Lysate from human lung in the 1) absence, 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung probed with TRAM antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: TRAM/TICAM2 Antibody [NBP2-24638] - Staining of human brain tissue probed with an isotype control antibody (top left) and TRAM antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-24638-img0003.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-32470] - Human breast cancer, using P2X7 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-32470-img0004.jpg "Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-32470] - Human breast cancer, using P2X7 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-32470] - Rat brain. P2X7 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-32470-img0007.jpg)
