Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated]

Images

 
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: NBP2-60656 ...read more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NBP2-60656] - Image from the DyLight 488 version of this antibody.
Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. ...read more
Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Donkey
Conjugate
Unconjugated
Concentration
2 mg/ml

Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] Summary

Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
This product is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional Array Products

Array NBP2-60656

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