Donkey anti-Goat IgG (H+L) Secondary Antibody [Rhodamine]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
Rhodamine

Donkey anti-Goat IgG (H+L) Secondary Antibody [Rhodamine] Summary

Immunogen
Full length native Goat IgG (purified).
Specificity
Goat IgG (H and L)
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Reactivity Notes

Goat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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