| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| CoraFluor 1 | NBP2-34728CL1 | |||
| FITC | NBP2-34728F | |||
| Janelia Fluor 525 | NBP2-34728JF525 | |||
| Janelia Fluor 669 | NBP2-34728JF669 | |||
| mFluor Violet 450 SE | NBP2-34728MFV450 | |||
| mFluor Violet 500 SE | NBP2-34728MFV500 | |||
| mFluor Violet 610 SE | NBP2-34728MFV610 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34728-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-00093SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-159SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| CoraFluor 1 | NBP2-34703CL1 | |||
| FITC | NBP2-34703F | |||
| Janelia Fluor 525 | NBP2-34703JF525 | |||
| Janelia Fluor 669 | NBP2-34703JF669 | |||
| mFluor Violet 450 SE | NBP2-34703MFV450 | |||
| mFluor Violet 500 SE | NBP2-34703MFV500 | |||
| mFluor Violet 610 SE | NBP2-34703MFV610 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34703-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76782-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74520 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34992-1mg | |||
| Carrier-Free | NBP2-34992-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
![Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-278202415212450.jpg "Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-298202414333619.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-29202410484842.jpg)
![Flow Cytometry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Flow cytometric analysis of NIH/3T3 cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32356, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-392024884678.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNF706) [NBP2-34303] - Immunofluorescence staining of HePG2 cells using TNF-alpha Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNF706-Immunocytochemistry-Immunofluorescence-NBP2-34303-img0002.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNF706) [NBP2-34303] - Immunofluorescence staining of HePG2 cells using TNF-alpha Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNF706) [NBP2-34303] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF-alpha Antibody (TNF706)](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNF706-Immunohistochemistry-Paraffin-NBP2-34303-img0003.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNF706) [NBP2-34303] - Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNF706-Immunohistochemistry-Paraffin-NBP2-34303-img0001.jpg)
![Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Western-Blot-NBP1-00093-img0009.jpg "Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00093] - The SR-AI/MSR antibody was tested in Raw cells at a 1:50 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00093-img0008.jpg)
![Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Western-Blot-NB400-159-img0005.jpg "Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NB400-159] - Antibody was tested in Raw cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Immunocytochemistry-Immunofluorescence-NB400-159-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (P/T2) [NBP2-34302] - Immunofluorescence staining of HePG2 cells using TNF-alphaMouse Monoclonal Antibody (P/T2) followed by goat anti-mouse IgG-CF488. Counterstained with RedDot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunocytochemistry-Immunofluorescence-NBP2-34302-img0003.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (P/T2) [NBP2-34302] - Immunofluorescence staining of HePG2 cells using TNF-alphaMouse Monoclonal Antibody (P/T2) followed by goat anti-mouse IgG-CF488. Counterstained with RedDot.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (P/T2) [NBP2-34302] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (P/T2).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunohistochemistry-Paraffin-NBP2-34302-img0002.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (P/T2) [NBP2-34302] - Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (P/T2).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunohistochemistry-Paraffin-NBP2-34302-img0001.jpg)
![Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0005.jpg "Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0001.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0003.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0004.jpg)
![Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Western-Blot-NBP1-76782-img0003.jpg "Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-76782] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-76782-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 ug/mL.Red: IRAK3 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-271120242013232.gif)
![Immunohistochemistry: IRAK3 Antibody [NBP1-76782] - Rat Liver tissue with IRAK-M antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunofluorescence-NBP1-76782-img0004.jpg)
![Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunohistochemical staining of rat liver tissue using IRAK3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-2711202419541592.jpg)
![Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Western-Blot-NBP2-45933-img0009.jpg "Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)")
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0005.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0001.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0002.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0003.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0004.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0006.jpg)
![SDS-Page: Human IFN-gamma Protein [NBP2-34992]](http://images.novusbio.com/fullsize/Human-IFN-gamma-Protein-SDS-Page-NBP2-34992-img0001.jpg "SDS-Page: Human IFN-gamma Protein [NBP2-34992]")
![Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-278202416331261.jpg "Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29820241492613.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunocytochemistry analysis of HeLa cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29202410334066.jpg)
![Flow Cytometry: FXR1 Antibody (JE40-57) [NBP3-32354] - Flow cytometric analysis of HeLa cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-392024884674.jpg)
![Western Blot: c-Maf Antibody [NB600-267] - Nuclear extract (6 ug) from HeLa cells transfected with empty vector or expression constructs for mouse MafA, mouse MafB or mouse cMaf. Antibody used at 0.4 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NB600-267-img0005.jpg "Western Blot: c-Maf Antibody [NB600-267] - Nuclear extract (6 ug) from HeLa cells transfected with empty vector or expression constructs for mouse MafA, mouse MafB or mouse cMaf. Antibody used at 0.4 ug/ml.")
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)