| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50328-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81085-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52685-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
![SDS-Page: Recombinant Human HSP90 beta Protein [NBP3-18320] - SDS-PAGE of 90kDa HSP90 beta protein (NBP3-18320). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HSP90 beta protein (NBP3-18320).](http://images.novusbio.com/fullsize/Recombinant Human HSP90 beta Protein-SDS-Page-NBP3-18320-img0001.jpg "SDS-Page: Recombinant Human HSP90 beta Protein [NBP3-18320] - SDS-PAGE of 90kDa HSP90 beta protein (NBP3-18320). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HSP90 beta protein (NBP3-18320).")
![Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-1992023141014.jpg "Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![<b>Biological Strategies Validation. </b>Western Blot: eNOS Antibody [NBP3-23210] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-19920231856933.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-Chimeric-Flow-Cytometry-NBP2-52685-img0002.jpg "Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (Matuzumab) - Chimeric - Azide and BSA Free [NBP2-52685] - Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzumab. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP2-52685 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52685, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52685_rabbit-monoclonal-egfr-antibody-matuzumab-chimeric-74202522141213.png)
![Western Blot: p53R2 Antibody [NBP2-19668] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP2-19668-img0002.jpg "Western Blot: p53R2 Antibody [NBP2-19668] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: p53R2 Antibody [NBP2-19668] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP2-19668-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP2-19668] - p53R2 antibody [N1C3] detects p53R2 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. p53R2 antibody [N1C3] (NBP2-19668) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19668_rabbit-polyclonal-p53r2-antibody-910202420131211.jpg)
![Western Blot: HspA4 Antibody [NBP2-16898] - Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HspA4-Antibody-Western-Blot-NBP2-16898-img0002.jpg "Western Blot: HspA4 Antibody [NBP2-16898] - Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HspA4 Antibody [NBP2-16898] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HspA4-Antibody-Immunohistochemistry-Paraffin-NBP2-16898-img0003.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA4 antibody [N3C2], Internal (NBP2-16898) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16898_rabbit-polyclonal-hspa4-antibody-western-blot-2611202518342613.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-159-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-159] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-159-img0003.jpg)
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody [NB200-159] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-p14ARF used at a dilution of 1:250.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Paraffin-NB200-159-img0001.jpg)
![Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PARP4-Antibody-Western-Blot-NBP1-06563-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563] - Human colon carcinoma. Antibody: Affinity purified rabbit anti- vPARP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PARP4-Antibody-Immunohistochemistry-Paraffin-NBP1-06563-img0006.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![<b>Biological Strategies Validation. </b>Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)