| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61641-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98484-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98742-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90660-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98267-50ul | |||
![Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Western-Blot-NB110-61641-img0007.jpg "Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0009.jpg)
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0010.jpg)
![Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/GBL-Antibody-Western-Blot-NBP2-98484-img0004.jpg "Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: GBL Antibody [NBP2-98484] - Immunochemical staining of human GBL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP2-98484-img0003.jpg)
![Immunoprecipitation: GBL Antibody [NBP2-98484] - GBL was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-GBL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GBL rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/GBL-Antibody-Immunoprecipitation-NBP2-98484-img0005.jpg)
![Western Blot: Endo G Antibody (JU31-30) [NBP2-75480] - Analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Endo-G-Antibody-JU31-30-Western-Blot-NBP2-75480-img0004.jpg "Western Blot: Endo G Antibody (JU31-30) [NBP2-75480] - Analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480] - Staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised](http://images.novusbio.com/fullsize/Endo-G-Antibody-JU31-30-Immunocytochemistry-Immunofluorescence-NBP2-75480-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480] - Staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Endo-G-Antibody-JU31-30-Immunocytochemistry-Immunofluorescence-NBP2-75480-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Endo G Antibody (JU31-30) [NBP2-75480] - Staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Endo-G-Antibody-JU31-30-Immunocytochemistry-Immunofluorescence-NBP2-75480-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-98742-img0002.jpg "Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-98742] - Immunofluorescence staining of GADD153/CHOP in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98742 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98742_rabbit-polyclonal-gadd153-chop-antibody-235202521525024.png)
![Western Blot: Nav1.5 Antibody [NBP2-86725] - WB Suggested Anti-SCN5A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/Nav1.5-Antibody-Western-Blot-NBP2-86725-img0002.jpg "Western Blot: Nav1.5 Antibody [NBP2-86725] - WB Suggested Anti-SCN5A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Muscle")
![Immunohistochemistry-Paraffin: Nav1.5 Antibody [NBP2-86725] - Rabbit Anti-SCN5A Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Membrane. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy2/3.](http://images.novusbio.com/fullsize/Nav1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-86725-img0001.jpg)
![Western Blot: Nav1.5 Antibody [NBP2-86725] - Host: Rabbit. Target Name: SCN5A. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Nav1.5-Antibody-Western-Blot-NBP2-86725-img0003.jpg)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0003.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90660 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90660_rabbit-monoclonal-protein-disulfide-isomerase-p4hb-antibody-023-235202514492359.png)
![Immunohistochemistry: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32946) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-298202413585575.jpg "Immunohistochemistry: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32946) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunocytochemistry analysis of MCF7 cells labeling DEC1 with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-29202410234280.jpg)
![Western blot analysis on different lysates with NBP3-32946 at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32946 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32946_-western-blot-639204595247363707.jpg)
![Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Western-Blot-NBP2-98267-img0005.jpg "Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0004.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0002.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0003.jpg)
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg "Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32894 at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: K-562 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32894 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_-western-blot-639204597482976311.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg "Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western blot analysis on different lysates with NBP3-32688 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32688 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_-western-blot-639204590074505803.jpg)