| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44500 | |||
| BSA Free | NBP2-52956-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54776-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97555-0.2mg | |||
| BSA Free | NBP1-97555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26446-50ul | |||
![Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Western-Blot-NBP2-52956-img0001.jpg "Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (CL0887) [NBP2-52956] - Staining of MCF7 cells using the Anti-AKT1 monoclonal antibody, showing specific staining in the nucleus, cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Immunocytochemistry-Immunofluorescence-NBP2-52956-img0002.jpg)
![Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/Snail-[p-Ser246]-Antibody-Western-Blot-NBP2-54776-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Snail [p Ser246] Antibody [NBP2-54776] - analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Snail [p Ser246] Antibody - BSA Free [NBP2-54776] - Immunofluorescence staining of methanol-fixed HuvEc cells using Snail (Phospho-Ser246) Antibody.](http://images.novusbio.com/fullsize/nbp2-54776_rabbit-polyclonal-snail-p-ser246-antibody-2711202419541572.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - HepG2 tissue lysate at a concentration of 0.5ug/ml.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP1-57949-img0005.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - HepG2 tissue lysate at a concentration of 0.5ug/ml.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-57949-img0004.jpg)
![Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ATM-Antibody-6P6C6-Western-Blot-NBP3-15838-img0002.jpg "Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: ATM Antibody (6P6C6) [ATM] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATM Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15838_rabbit-atm-mab-6p6c6-52202517172899.jpg)
![Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-5220251265374.jpg "Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273] - Confocal imaging of RAW 264.7 cells (treated with LPS) and RAW 264.7 cells(untreated) using iNOS Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16273_rabbit-inos-mab-3l0u6-52202511392210.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: nNOS Antibody [NBP1-97555] - Analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-97555-img0003.jpg "Western Blot: nNOS Antibody [NBP1-97555] - Analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.")
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-97555] - Human brain, cortex tissue. Dilution at 20ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-97555-img0002.jpg)
![Western Blot: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Western blot analysis of TIMP-3 on different lysates with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Human liver tissue lysate (negative) Lane 4: Rat placenta tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33023_rabbit-timp-3-mab-psh04-41-288202412493444.jpg "Western Blot: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Western blot analysis of TIMP-3 on different lysates with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Human liver tissue lysate (negative) Lane 4: Rat placenta tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TIMP-3 Antibody (PSH04-41) [NBP3-33023] - Immunocytochemistry analysis of HeLa cells labeling TIMP-3 with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP-3 antibody (NBP3-33023) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33023_rabbit-timp-3-mab-psh04-41-2920241192391.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Immunohistochemistry: OS9 Antibody (26H3) [NBP3-26446] - Image of OS9 Antibody (26H3) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26446_rabbit-os9-mab-26h3-2722024857333.jpg "Immunohistochemistry: OS9 Antibody (26H3) [NBP3-26446] - Image of OS9 Antibody (26H3) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: OS9 Antibody (26H3) [NBP3-26446] - Positive Western Blot detected in: HL-60 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue. All lanes: OS9 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76, 70, 68, 74, 66, 64, 70, 61 kDa Observed band size: 72-95 kDa](http://images.novusbio.com/fullsize/nbp3-26446_rabbit-os9-mab-26h3-282202411472140.jpg)