| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03693-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61733-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37249-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
![Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/STEP-Antibody-Western-Blot-NBP3-03693-img0001.jpg "Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of Neuro-2a cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-52202519302959.jpg)
![Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of SH-SY5Y cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03693_rabbit-polyclonal-step-antibody-5220251931349.jpg)
![Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Western blot analysis of extracts of various cell lines, using MKK3/MEK3 Rabbit mAb (NBP3-16163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-8U2X3-Western-Blot-NBP3-16163-img0004.jpg "Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Western blot analysis of extracts of various cell lines, using MKK3/MEK3 Rabbit mAb (NBP3-16163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Confocal imaging of C6 cells using MKK3/MEK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16163_rabbit-mkk3-mek3-mab-8u2x3-72202512435370.jpg)
![Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Confocal imaging of NIH/3T3 cells using MKK3/MEK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16163_rabbit-mkk3-mek3-mab-8u2x3-72202512432014.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Western-Blot-NBP1-04346-img0007.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP1-04346-img0006.jpg)
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Flow-Cytometry-NBP1-04346-img0008.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-9A7X4-Western-Blot-NBP3-16720-img0001.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Western blot analysis of various lysates, using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-52202511411497.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-5220251215491.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Western-Blot-NBP3-16482-img0003.jpg "Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of NIH-3T3 cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0001.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-685] - Formalin fixed paraffin embedded human placenta stained with VEGFR1/Flt-1 antibody (NB100-685).](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-685-img0004.jpg "Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-685] - Formalin fixed paraffin embedded human placenta stained with VEGFR1/Flt-1 antibody (NB100-685).")
![Immunohistochemistry: VEGF R1/Flt-1 Antibody [NB100-685] - Formalin fixed paraffin embedded human angiosarcoma stained with Flt-1.](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Immunohistochemistry-NB100-685-img0003.jpg)
![Western Blot: CHRND Antibody (1H1F9) [NBP2-61733] - Analysis using CHRND mAb against HEK293 (1) and CHRND (AA: extra 22-245)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CHRND-Antibody-1H1F9-Western-Blot-NBP2-61733-img0004.jpg "Western Blot: CHRND Antibody (1H1F9) [NBP2-61733] - Analysis using CHRND mAb against HEK293 (1) and CHRND (AA: extra 22-245)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: CHRND Antibody (1H1F9) [NBP2-61733] - Analysis of SK-N-SH cells using CHRND mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CHRND-Antibody-1H1F9-Flow-Cytometry-NBP2-61733-img0002.jpg)
![Western Blot: CHRND Antibody (1H1F9) [NBP2-61733] - Analysis using CHRND mAb against human CHRND (AA: extra 22-245) recombinant protein. (Expected MW is 52.2 kDa)](http://images.novusbio.com/fullsize/CHRND-Antibody-1H1F9-Western-Blot-NBP2-61733-img0003.jpg)
![Western Blot: CHRND Antibody (1H1F9) [NBP2-61733] - Analysis using CHRND mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/CHRND-Antibody-1H1F9-Western-Blot-NBP2-61733-img0005.jpg)
![ELISA: CHRND Antibody (1H1F9) [NBP2-61733] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CHRND-Antibody-1H1F9-ELISA-NBP2-61733-img0001.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0003.jpg "Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Flow-Cytometry-NBP2-37249-img0004.jpg)
![Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-Western-Blot-NBP2-37249-img0002.jpg)
![ELISA: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-6C3F6-ELISA-NBP2-37249-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-288202412442966.jpg "Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32946) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-298202413585575.jpg)
![Immunocytochemistry/ Immunofluorescence: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunocytochemistry analysis of MCF7 cells labeling DEC1 with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-29202410234280.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-278202415242692.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29820241420924.jpg)
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunocytochemistry analysis of THP-1 cells labeling Carbonic Anhydrase II/CA2 with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29202410334053.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)