| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89890-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9216-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34628-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80706 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1298SS | |||
| Azide and BSA Free | NBP2-80567 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80709 | |||
![Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Western-Blot-NBP2-89890-img0003.jpg "Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunohistochemistry-Paraffin-NBP2-89890-img0001.jpg)
![Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate 2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunoprecipitation-NBP2-89890-img0004.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human ORP150/HSP12A in human colon carcinoma with NBP2-89890 (1:200).](http://images.novusbio.com/fullsize/nbp2-89890_rabbit-monoclonal-orp150-hsp12a-antibody-201-235202514492364.png)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89890 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89890_rabbit-monoclonal-orp150-hsp12a-antibody-201-23520251664729.png)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0005.jpg "Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Immunohistochemistry-Paraffin-NBP2-67552-img0002.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0004.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0003.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0001.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: Clusterin/APOJ Antibody (Hs-3) [NB500-552] - Analysis of clusterin in human seminal plasma using anti-clusterin (Hs-3) purified.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-Hs-3-Western-Blot-NB500-552-img0001.jpg "Western Blot: Clusterin/APOJ Antibody (Hs-3) [NB500-552] - Analysis of clusterin in human seminal plasma using anti-clusterin (Hs-3) purified.")
![Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-27820241631652.jpg "Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63/TP73L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32703, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-298202413585578.jpg)
![Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (SC06-31) [NBP3-32703] - ICC staining of p63/TP73L in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32703, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-2920241192350.jpg)
![Flow Cytometry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Flow cytometric analysis of p63/TP73L was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32703, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-29202416453240.jpg)
![Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-Western-Blot-NBP1-32734-img0006.jpg "Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Analysis of paraformaldehyde-fixed A431, using UBE2D1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32734-img0009.jpg)
![Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse lung. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-Immunohistochemistry-Paraffin-NBP1-32734-img0008.jpg)
![Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - TOV-112D xenograft, using UBE2D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-Immunohistochemistry-Paraffin-NBP1-32734-img0005.jpg)
![Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse prostate. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-Immunohistochemistry-Paraffin-NBP1-32734-img0007.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Analysis of HepG2, Daudi and MOLT-4 cells lysates with 0.5 ug/ml of hLDHA/LDHB Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hLDHA/LDHB at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2066A-Western-Blot-MAB9216-img0001.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Analysis of HepG2, Daudi and MOLT-4 cells lysates with 0.5 ug/ml of hLDHA/LDHB Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hLDHA/LDHB at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Lactate Dehydrogenase A/LDHA was detected in immersion fixed PC3 cell using Lactate Dehydrogenase A/LDHA Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2066A-Immunocytochemistry-MAB9216-img0002.jpg)
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)
![Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Analysis of mouse thymus tissue using EBI3 antibody at a concentration of 1 ug/ml.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Western-Blot-NBP2-03940-img0007.jpg "Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Analysis of mouse thymus tissue using EBI3 antibody at a concentration of 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Figure A: Intracellular analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Flow-Cytometry-NBP2-03940-img0005.jpg)
![Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (10J803) [NBP2-03940] - Western blot testing of mouse EBI3 monoclonal antibody at A) 0.1 ug/ml on recombinant protein and B) 3 ug/ml on recombinant mouse IL-35 protein. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-27-IL-35-EBI3-Subunit-Antibody-10J803-Western-Blot-NBP2-03940-img0006.jpg)
![Western Blot: AKT3 Antibody (66C1247) [NB600-1298] - Analysis for AKT3 using NB600-1298 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate.](http://images.novusbio.com/fullsize/AKT3-Antibody-66C1247-Western-Blot-NB600-1298-img0003.jpg "Western Blot: AKT3 Antibody (66C1247) [NB600-1298] - Analysis for AKT3 using NB600-1298 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate.")
![Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P10D3-Western-Blot-NBP2-03943-img0003.jpg "Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943] - Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 2 ug/10^6 cells.](http://images.novusbio.com/fullsize/EBI3-Antibody-5P10D3-Flow-Cytometry-NBP2-03943-img0002.jpg)