| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38409-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95255-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
![Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NB100-1400-img0004.jpg "Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1400-img0005.jpg)
![Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NB100-1400] - Staining of paraffin embedded Human Testis. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1400-img0002.jpg)
![Flow Cytometry: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Flow-Cytometry-NB100-1400-img0006.jpg)
![Immunohistochemistry: AMPK gamma 1 Antibody [NBP3-38409] - Immunohistochemistry analysis of paraffin-embedded Rat brain using AMPK gamma 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38409_rabbit-ampk-gamma-1-pab-912202415152879.jpg "Immunohistochemistry: AMPK gamma 1 Antibody [NBP3-38409] - Immunohistochemistry analysis of paraffin-embedded Rat brain using AMPK gamma 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: AMPK gamma 1 Antibody [NBP3-38409] - Immunohistochemistry analysis of paraffin-embedded Human stomach using AMPK gamma 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38409_rabbit-ampk-gamma-1-pab-912202415143668.jpg)
![Immunocytochemistry/ Immunofluorescence: AMPK gamma 1 Antibody [NBP3-38409] - Immunofluorescence analysis of MCF-7 cells using AMPK gamma 1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38409_rabbit-ampk-gamma-1-pab-912202414112812.jpg)
![Immunohistochemistry: AMPK gamma 1 Antibody [NBP3-38409] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using AMPK gamma 1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38409_rabbit-ampk-gamma-1-pab-91220241519577.jpg)
![Western Blot: AMPK gamma 1 Antibody [NBP3-38409] - Western blot analysis of various lysates using AMPK gamma 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38409_rabbit-ampk-gamma-1-pab-912202415152818.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Western-Blot-NBP3-15357-img0002.jpg "Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357] - Immunohistochemistry of paraffin-embedded Mouse kidney using Catalase antibody (NBP3-15357) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Immunohistochemistry-Paraffin-NBP3-15357-img0001.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [Catalase] - Western blot analysis of lysates from Hep G2 cells using Catalase Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517213587.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-5220251728490.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517223975.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517172853.jpg)
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Sodium Potassium ATPase Alpha 1 Antibody-Western Blot-NBP2-95255-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded mouse kidney using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human liver cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human lung cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody - Azide and BSA Free [Sodium Potassium ATPase Alpha 1] - Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp2-95255_rabbit-polyclonal-sodium-potassium-atpase-alpha-1-antibody-5220251640221.jpg)
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Western-Blot-NBP2-37610-img0002.jpg "Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody (5G3) [NBP2-37610] - Analysis of Hela cells using CREB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunocytochemistry-Immunofluorescence-NBP2-37610-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (5G3) [NBP2-37610] - Analysis of prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunohistochemistry-NBP2-37610-img0004.jpg)
![ELISA: CREB Antibody (5G3) [NBP2-37610] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-ELISA-NBP2-37610-img0001.jpg)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")
![Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-ECE-2-Immunohistochemistry-Frozen-NBP1-42312-img0001.jpg "Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.")