| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61620 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75924SS | |||
| Azide and BSA Free | NBP2-80828 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78782SS | |||
| Azide and BSA Free | NBP2-80714 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/nbp3-43847_mouse-cox4-mab-cl3515-azide-and-bsa-free-282202512104221.jpg "Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP2-14113-img0002.jpg "Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP2-14113-img0001.jpg)
![Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Simple Western lane view shows a specific band for ORP150/HSP12A in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Simple-Western-NBP2-14113-img0003.jpg)
![Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Electropherogram image of the corresponding Simple Western lane view. ORP150/HSP12A antibody was used at 1:25 dilution on HEL lysate(s) respectively.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Simple-Western-NBP2-14113-img0004.jpg)
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Western-Blot-NBP2-78782-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) at 3 ug/mL for 1 hour at room temperature followed by incubation with an anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Immunohistochemistry-Paraffin-NBP2-78782-img0002.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody detects GSK-3 beta protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK-3 beta stained by GSK-3 beta antibody (NBP3-13679) diluted at 1:500.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunocytochemistry-Immunofluorescence-NBP3-13679-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0003.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0001.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-212024941427.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-261120251839316.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunohistochemistry-paraffin-26112025195717.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-26112025199124.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518224316.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518463713.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-261120251818503.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![ICC/IF: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13144_rabbit-tor-mtor-p-ser-2448-pab-immunocytochemistry-immunofluorescence-126202482926..jpg)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0002.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-59190-img0003.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0004.jpg)