| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96872-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61852-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-266SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Azide and BSA Free | NBP2-34809-0.1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-455SS | |||
![Western Blot: HSP90 Antibody (D7A) [NB110-96872] - Western Blot analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha (NB110-96872). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-96872) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-D7A-Western-Blot-NB110-96872-img0007.jpg "Western Blot: HSP90 Antibody (D7A) [NB110-96872] - Western Blot analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha (NB110-96872). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-96872) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP90 Antibody (D7A) [NB110-96872] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha (NB110-96872). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-96872) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-Antibody-D7A-Immunohistochemistry-NB110-96872-img0008.jpg)
![Immunohistochemistry: HSP90 Antibody (D7A) [NB110-96872] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha (NB110-96872). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-96872) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-Antibody-D7A-Immunohistochemistry-NB110-96872-img0009.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0006.jpg "Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Immunohistochemistry-NBP2-61852-img0004.jpg)
![Flow Cytometry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Flow-Cytometry-NBP2-61852-img0002.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against human *** (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0005.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0007.jpg)
![Immunohistochemistry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Immunohistochemistry-NBP2-61852-img0003.jpg)
![ELISA: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-ELISA-NBP2-61852-img0001.jpg)
![Immunohistochemistry: BNIP3L Antibody (JE59-98) [NBP3-32087] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32087) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-2982024143525.jpg "Immunohistochemistry: BNIP3L Antibody (JE59-98) [NBP3-32087] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32087) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody (JE59-98) [NBP3-32087] - Immunocytochemistry analysis of HeLa cells labeling BNIP3L with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-292024119232.jpg)
![Flow Cytometry: BNIP3L Antibody (JE59-98) [NBP3-32087] - Flow cytometric analysis of HeLa cells labeling BNIP3L.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32087, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-29202416453213.jpg)
![Western blot analysis on different lysates with NBP3-32087 at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32087 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32087_-western-blot-639204597483819095.jpg)
![Western blot analysis on different lysates with NBP3-32087 at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 5 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32087 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32087_-western-blot-639204598086384164.jpg)
![Western Blot: Beclin 1 Antibody [NB500-266] - Detection of Beclin 1 in liver lysates using NB500-266. (1) mouse liver and (2) human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Western-Blot-NB500-266-img0002.jpg "Western Blot: Beclin 1 Antibody [NB500-266] - Detection of Beclin 1 in liver lysates using NB500-266. (1) mouse liver and (2) human liver.")
![Western Blot: AIP/ARA9 Antibody [NBP1-31347] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ARA9 antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-Western-Blot-NBP1-31347-img0011.jpg "Western Blot: AIP/ARA9 Antibody [NBP1-31347] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ARA9 antibody [N1C3] diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: AIP/ARA9 Antibody [NBP1-31347] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31347-img0006.jpg)
![Immunohistochemistry-Paraffin: AIP/ARA9 Antibody [NBP1-31347] - Human lung carcinoma. ARA9 antibody [N1C3] diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-Immunohistochemistry-Paraffin-NBP1-31347-img0010.jpg)
![Western Blot: AIP/ARA9 Antibody [NBP1-31347] - Mouse brain whole cell lysate (50 ug), stained with anti-AIP/ARA9 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-Western-Blot-NBP1-31347-img0007.jpg)
![Western Blot: AIP/ARA9 Antibody [NBP1-31347] - A. 30 ug HeLa whole cell lysate/extract, B. 30 ug Molt-4 whole cell lysate/extract, C. 30 ug Raji whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:5000.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-Western-Blot-NBP1-31347-img0009.jpg)
![Western Blot: Vimentin Antibody (SPM576) [NBP2-32910] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Western-Blot-NBP2-32910-img0002.jpg "Western Blot: Vimentin Antibody (SPM576) [NBP2-32910] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) [NBP2-32910] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Immunohistochemistry-Paraffin-NBP2-32910-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/COX-2-Antibody-Western-Blot-NB110-1948-img0001.jpg "Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.")
![Western Blot: VG5Q Antibody [NB100-455] - Murine VG5Q detected in mouse skin protein extracts.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Western-Blot-NB100-455-img0002.jpg "Western Blot: VG5Q Antibody [NB100-455] - Murine VG5Q detected in mouse skin protein extracts.")
![Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.](http://images.novusbio.com/fullsize/AMPK-beta-1-Antibody-2C3-Western-Blot-H00005564-M02-img0007.jpg "Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AMPK-beta-1-Antibody-2C3-Immunocytochemistry-Immunofluorescence-H00005564-M02-img0005.jpg)
![Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AMPK-beta-1-Antibody-2C3-Sandwich-ELISA-H00005564-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432892.jpg "Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249422641.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-212024942543.jpg)
![Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249393587.jpg)
![Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432876.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-western-blot-261120251830385.jpg)
