| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-21690-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41622SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07034SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27184SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88558-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85814-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Western-Blot-NBP1-21690-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Immunocytochemistry-Immunofluorescence-NBP1-21690-img0005.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Western-Blot-NB600-1164-img0031.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Protein Disulfide Isomerase/P4HB using monoclonal antibody shows staining in A549 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0027.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0030.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Staining of rat epithelial cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of NS-1 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis shows staining in HMVEC Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0026.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Normal deparaffinized Human tonsil tissue tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0028.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human liver tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0029.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of K562 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0022.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0023.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Western Blot: SAT1 Antibody [NB110-41622] - Detection of SAT1 in human SAT1 transfected lysate.](http://images.novusbio.com/fullsize/SAT1-Antibody-Western-Blot-NB110-41622-img0004.jpg "Western Blot: SAT1 Antibody [NB110-41622] - Detection of SAT1 in human SAT1 transfected lysate.")
![Immunohistochemistry: SAT1 Antibody [NB110-41622] - IHC analysis of SAT1 in mouse seminal vesical (left) and prostate (right) usind DAB with hematoxylin counterstain,](http://images.novusbio.com/fullsize/SAT1-Antibody-Immunohistochemistry-NB110-41622-img0006.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP1-07034-img0004.jpg "Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.")
![Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of MCP1 in Hela whole cell lysates.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP1-07034-img0003.jpg)
![Western Blot: Snail Antibody [NBP2-27184] - Analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma, 6nM TNF alpha, and 3nM IL1 beta). Expression of Snail (NBP2-27184SS), E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP2-27184-img0006.jpg "Western Blot: Snail Antibody [NBP2-27184] - Analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma, 6nM TNF alpha, and 3nM IL1 beta). Expression of Snail (NBP2-27184SS), E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Snail Antibody [NBP2-27184] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with SNAIL (NBP2-27184) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with DyLight 550 (Red). Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Snail-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27184-img0005.jpg)
![Western Blot: Snail Antibody [NBP2-27184] - Analysis of Snail in human MCF7 cell lysate using (1) prebleed at 1:5000 dilution and (2) this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Snail-Antibody-Western-Blot-NBP2-27184-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: BNIP3L Antibody [NBP1-88558] - UCB-hMSCs were exposed to normoxia or hypoxia. (D) The mRNA expressions of PINK1, BNIP3, NIX and FUNDC1 were analyzed by quantitative real-time PCR (qPCR). n = 5. (E) The protein expressions of PINK1, BNIP3, NIX and B-actin were assessed by western blot. n = 4. *p < 0.05 versus control. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Western-Blot-NBP1-88558-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BNIP3L Antibody [NBP1-88558] - Vehicle or RU 486 (5 mg/kg) injected mice were presented with/without corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha NBP1-04676) and NIX (BNIP3L NBP1-88558) were visualized via western blotting. Loading control is beta-actin. n = 5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BNIP3L Antibody-Western Blot-NBP1-88558-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3L Antibody [NBP1-88558] - Staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88558-img0006.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0008.jpg)
![Western Blot: BNIP3L Antibody [NBP1-88558] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121937.jpg)
![Western Blot: BNIP3L Antibody [NBP1-88558] - GR-dependent downregulation of NIX expression via PGC1 alpha .a, b Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 24 h, respectively. NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. c SH-SY5Y cells were incubated with cortisol for 6 h. The mRNA expression levels of genes associated with nuclear receptors & coregulators were assessed by RT2 Profiler PCR array. Heat maps with hierarchical clustering were acquired by using the GeneGlobe Data analysis Center on Qiagen website. n = 3. d A thousand base pair upstream of the first codon of the PPARGC1A was described & the putative GRE binding sequence was emphasized with yellow labeling. e SH-SY5Y cells were incubated with cortisol for 6 h. DNA was immunoprecipitated with IgG, RNA polymerase (RNAPol), & glucocorticoid receptor (GR) antibody. The immunoprecipitation & input samples were amplified with primers of GAPDH & PPARGC1A gene. n = 5. All blots are representative. n = 3 or 5 from independent experiments with two technical replicates each, respectively. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted in Fig. 6a, b. Two-sided unpaired student’s t test was conducted in Fig. 6e. ** indicates p < 0.01 versus control. ## indicates p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121914.jpg)
![Western Blot: BNIP3L Antibody [NBP1-88558] - GR-dependent downregulation of NIX expression via PGC1 alpha .a, b Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 24 h, respectively. NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. c SH-SY5Y cells were incubated with cortisol for 6 h. The mRNA expression levels of genes associated with nuclear receptors & coregulators were assessed by RT2 Profiler PCR array. Heat maps with hierarchical clustering were acquired by using the GeneGlobe Data analysis Center on Qiagen website. n = 3. d A thousand base pair upstream of the first codon of the PPARGC1A was described & the putative GRE binding sequence was emphasized with yellow labeling. e SH-SY5Y cells were incubated with cortisol for 6 h. DNA was immunoprecipitated with IgG, RNA polymerase (RNAPol), & glucocorticoid receptor (GR) antibody. The immunoprecipitation & input samples were amplified with primers of GAPDH & PPARGC1A gene. n = 5. All blots are representative. n = 3 or 5 from independent experiments with two technical replicates each, respectively. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted in Fig. 6a, b. Two-sided unpaired student’s t test was conducted in Fig. 6e. ** indicates p < 0.01 versus control. ## indicates p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121960.jpg)
![Western Blot: BNIP3L Antibody [NBP1-88558] - Effects of hypoxia on mitophagy regulator expressions & mitophagy in UCB-hMSCs. (A) UCB-hMSCs incubated w/ various times of hypoxia (0–48 h). Cells stained w/ Mitotracker™. n = 6 (magnification, ×1,200). Scale bars, 50 µm. (B) The expressions of COX4 & beta -actin detected by western blot. n = 4. (C) UCB-hMSCs exposed to 24 h of normoxia or hypoxia. Cells immuno-stained w/ COX4 & LC3B-specific antibodies (magnification, ×600). Scale bars, 37.5 µm. (D) The mRNA expressions of PINK1, BNIP3, NIX & FUNDC1 analyzed by quantitative real-time PCR (qPCR). n = 5. (E) The protein expressions of PINK1, BNIP3, NIX & beta -actin assessed by western blot. n = 4. (F, G) siRNAs of PINK1, BNIP3, NIX or non-targeting (NT) transfected to UCB-hMSCs prior to hypoxia treatment for 24 h. COX4 & beta -actin expressions assessed by western blot. n = 4 (F). Cells immunostained w/ COX4 & PI. n = 3 (magnification, ×400). All scale bars, 50 µm. COX4 fluorescence intensity analyzed by luminometer. n = 5 (G). (H) BNIP3 siRNA transfected to UCB-hMSCs prior to hypoxia treatment for 24 h. Cells stained w/ Mitotracker™. n = 6 (magnification, ×1,200). Scale bars, 50 µm. (I) BNIP3, beta -tubulin & COX4 w/ cytosol & mitochondrial fractionized samples detected by western blot. (J) Cells incubated w/ hypoxia or normoxia for 24 h. Cells immuno-stained w/ BNIP3 & LC3B-specific antibodies. (magnification, ×600). Scale bars, 37.5 µm. Western blot data normalized by beta -actin, & qPCR data normalized by ACTB mRNA expression level. Quantitative data are presented as a mean ± S.E.M. All blot & confocal images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121983.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416113531.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114791.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114754.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121953.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-31020241613647.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85814-img0021.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0027.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0025.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0026.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0028.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0007.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)