| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47910-0.1mg | |||
| IHC-Prediluted | NBP2-48332 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81943-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76454SS | |||
| Azide and BSA Free | NBP2-80767 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70954 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
![Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0003.jpg "Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.")
![Western Blot: ATM Antibody [NB100-678] - MDA-MB-231 and MCF-7 whole cell lysates were loaded with 50 ug/lane. 8% SDS-PAGE. ATM Antibody (NB100-678) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0009.jpg)
![Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ATM-Antibody-Immunohistochemistry-NB100-678-img0007.jpg)
![Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0008.jpg)
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-NBP2-44568-img0005.jpg "Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Placenta stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunofluorescence-NBP2-44568-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - Immunofluorescence staining of retained human SVF adjacent to the site of cell-seeded scaffold transplantation. (A) Human SVF are visualized using human specific nuclear matrix antigen (NuMA). Immunofluorescence imaging of human-specific antibodies targeting CD34 (B) & CD31 (C). Scale bar: 50 μm (A), 100 μm (B,C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44568_mouse-monoclonal-cd34-antibody-hpca1-763-31020241535640.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81943-img0006.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943")
![Immunoprecipitation: GSK-3 alpha Antibody [NB100-81943] - Human HaCaT keratinocyte lysates with GSK3 alpha Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunoprecipitation-NB100-81943-img0010.jpg)
![Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Western-Blot-NB100-81943-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-81943(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81943_rabbit-polyclonal-gsk-3-alpha-antibody-10320252332100.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0009.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-H00005034-B01P-img0005.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0006.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in transfected 293T cell line by P4HB polyclonal antibody. Lane 1: P4HB transfected lysate(55.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0008.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0007.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Absence of TRIC-B alters expression of collagen-interacting proteins.(A) Quantitative RT-PCR of fibroblast transcripts encoding collagen, modifying enzymes & chaperones. Proband cells (P1, P2, & P3) demonstrate decreased expression of COL1A1 & FKBP10 (FKBP65), & increased expression of PLOD1 (LH1) & HSPA5 (BiP/GRP78) versus control fibroblasts (C). (B) Decreased expression of PLOD2 (LH2), FKBP10 (FKBP65) & PPIB (CyPB) in P2 osteoblasts versus control osteoblasts (C). (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes & chaperones in proband & control cells. Proband fibroblasts show increases in PDI, LH1, LH2 & BiP protein levels. CyPB & FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts & osteoblasts. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006156), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005034-b01p_mouse-polyclonal-protein-disulfide-isomerase-p4hb-antibody-31020241548512.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0001.jpg "Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Human MDA MB 231 cells. 50 ug cell lysate per lane. 10% SDS-PAGE. HIF-2 alpha antibody at 1:1000 in 5% milk. HRP-conjugated donkey anti-rabbit IgG polyclonal secondary antibody at 1:2000 in 5% milk. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0003.jpg)
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Analysis of an FFPE tissue section of the human renal carcinoma using 1:100 dilution of HIF-2 alpha antibody (NBP2-76454). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the nuclear with minor cytoplasmic staining.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Immunohistochemistry-Paraffin-NBP2-76454-img0002.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Western-Blot-NBP2-02190-img0011.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP2-02190-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Flow-Cytometry-NBP2-02190-img0010.jpg)
![Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Western-Blot-NBP2-02190-img0009.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0006.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)