| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76657-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88558-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29626SS | |||
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0010.jpg "Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL")
![Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Immunohistochemistry-NBP1-76657-img0005.jpg)
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) cell lysates. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0008.jpg)
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (A: 0.5 ug/mL, B: 0.5 ug/mL, C: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0009.jpg)
![Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76657_rabbit-polyclonal-endo-g-antibody-imgenex-img-5565-1-271120242040558.gif)
![Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 15 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76657_rabbit-polyclonal-endo-g-antibody-imgenex-img-5565-1-2711202420243350.jpg)
![Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling Endo G with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76657_rabbit-polyclonal-endo-g-antibody-imgenex-img-5565-1-271120242092060.gif)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0006.jpg "Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.")
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human breast carcinoma using AKT1 antibody at 1:50. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of extracts from COS cells using AKT1 antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human endometrial cancer using AKT1 antibody at 1:25. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77683-img0005.jpg "Immunocytochemistry/Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: BNIP3 Antibody [NBP1-77683] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-NBP1-77683-img0004.jpg)
![Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Flow-Cytometry-NBP1-77683-img0007.jpg)
![Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Flow-Cytometry-NBP1-77683-img0006.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BNIP3L Antibody [NBP1-88558] - Staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88558-img0006.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416113531.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114791.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114754.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121953.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-31020241613647.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0009.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0001.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0004.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PCP (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0017.jpg "Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of human hepatoma. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of mouse skin. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of rat hind brain. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0003.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [1055] diluted at 1:500. Blue: DAPI](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Frozen-NBP2-43646-img0009.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251820022.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-2611202518255016.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunohistochemistry-frozen-2611202518333613.jpg)
![Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251826138.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250.Red: GFAP, stained by GFAP antibody diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunocytochemistry-immunofluorescence-2611202519365.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0015.jpg "Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human alveolar cell.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0011.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0012.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - WB analysis of GLT1 in Hep3B WCL. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0013.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Human Fetal Brain lysates.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0014.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human Brain, cortex tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0010.jpg)
![Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.](http://images.novusbio.com/fullsize/SNAIL-Antibody-Western-Blot-NBP2-29626-img0001.jpg "Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)