| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77666SS | |||
| Azide and BSA Free | NBP2-80718 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-530SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78964SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-527SS | |||
![Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Western-Blot-NB100-77666-img0001.jpg "Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunocytochemistry-Immunofluorescence-NB100-77666-img0004.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the pseudostratified columnar epithelial cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunohistochemistry-Paraffin-NB100-77666-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cytometry-NB100-77666-img0002.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cell-Surface-NB100-77666-img0003.jpg)
![Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Western-Blot-NB100-530-img0009.jpg "Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Immunohistochemical analyses of VEGFR2, Ang-2, vWF and CD34 in the peri-infarct area of the ischemic brain. Representative merged images counterstained with DAPI (nuclear staining) (blue). Ang-2, VEGFR2, vWF and CD34 antibodies are represented by the green color (arrows). Image collected and cropped by Citeab from the following publication (A Short Bout of Exercise Prior to Stroke Improves Functional Outcomes by Enhancing Angiogenesis. Neuromolecular Med (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-530-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - V6.5 mouse embryonic stem cells (cat# NBP1-41162) stained with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-530-img0008.jpg)
![Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Top: relative expression of CD36, GLUT2, & VEGFR2 in duodenum; n = 6–8. All values are expressed as model-based means, with error bars representing the confidence intervals (95% CI). *P value < 0.05. Bottom: Western blots of CD36 (75 kDa), GLUT2 (55 kDa), & VEGFR2 (105 kDa). Animals without a detectable band were excluded from the analysis. HIIT, high-intensity interval training; MICT, moderate-intensity continuous training; CON, control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28183816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-530_rabbit-polyclonal-vegfr2-kdr-flk-1-antibody-310202415334928.jpg)
![Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Western-Blot-H00005688-M01-img0018.jpg "Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Immunocytochemistry-Immunofluorescence-H00005688-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Immunohistochemistry-Paraffin-H00005688-M01-img0013.jpg)
![Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Analysis of PSMA7 expression in HeLa.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Western-Blot-H00005688-M01-img0016.jpg)
![Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Western-Blot-H00005688-M01-img0017.jpg)
![Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Immunohistochemistry-Paraffin-H00005688-M01-img0011.jpg)
![Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Immunoprecipitation-H00005688-M01-img0014.jpg)
![Sandwich ELISA: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PSMA7-Antibody-1A10-3G12-Sandwich-ELISA-H00005688-M01-img0015.jpg)
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-78964-img0015.jpg "Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78964-img0017.jpg)
![Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-NBP1-78964-img0016.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0011.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12")
![Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human Placenta Antibody Concentration: 5 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-NBP1-55415-img0006.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human pancreatic cancer cell line MiaPaca-2 and Panc-1, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0001.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - 721_B Whole Cell, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0002.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Antibody Titration: 1 ug/ml Human 721_B.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0007.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Antibody Titration: 1 ug/ml Human Raji.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0008.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Lanes: 1. 30 ug MiaPaca-2 cell lysate 2. 30 ug Panc-1 cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 4000 Gene name: LDHB.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0010.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Lane A: Human Fetal Lung Lane B: Human HCT116 Lane C: Human 293T, Antibody Dilution: 0.2 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0009.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Western-Blot-NB120-5455-img0013.jpg "Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0008.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an HSP90 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Staining of human colon cancer tissue sections.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0010.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0011.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0026.jpg "Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0019.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:400 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0025.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0021.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0022.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0018.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0024.jpg)
![Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing CREB overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:1000 overnight rotating at 4C. Membranes were then washed in TBST and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunoprecipitation-NB100-74393-img0023.jpg)
![Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Simple-Western-NB910-95609-img0005.jpg "Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. Anti-Beclin-1 Antibody NB500-249](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95609] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95609-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95609] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production using NB500-249. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Knockdown-Validated-NB910-95609-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody Pack [NB910-95609] - Beclin 1 antibody (NB500-249) was tested in HeLa cells with Dylight 488 (green, left panel). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red), shown in the overlay (right panel)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-95609-img0010.jpg)
![Beclin 1 Antibody Pack [NB910-95609] - Detecting Beclin 1 using NB500-249 in Pheochromocytes of the Adrenal Medulla at 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95609-img0011.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Detection of Beclin 1 in liver lysates using NB500-266. (1) mouse liver and (2) human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0014.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Analysis of Beclin1 using NB500-249. Lane 1: human brain. Lane 2: mouse brain.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0006.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Western-Blot-NB100-527-img0005.jpg "Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-527-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0009.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0008.jpg)
