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![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - IHC-P staining of FFPE human breast cancer tissue sections using NBP2-61475 at a 1:400 dilution.](http://images.novusbio.com/fullsize/nbp2-61475_rabbit-akt1-p-ser473-mab-rm251-immunohistochemistry-paraffin-189202523512118.png "Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - IHC-P staining of FFPE human breast cancer tissue sections using NBP2-61475 at a 1:400 dilution.")
![Western Blot: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - WB of lysates from 293 cells either grown in medium with serum, serum starved, or insulin treated using NBP2-61475 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp2-61475_rabbit-akt1-p-ser473-mab-rm251-western-blot-18920252352110.png)
![Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-24620241332459.png "Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.")
![Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430] - Mouse skeletal muscle tissue lysate using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-immunohistochemistry-paraffin-189202523374021.png)
![Western Blot: Semaphorin 5A Antibody [NBP2-20291] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/SEMA5A-Antibody-Western-Blot-NBP2-20291-img0001.jpg "Western Blot: Semaphorin 5A Antibody [NBP2-20291] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NBP2-20291] - Gastric cancer, using Semaphorin 5A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/SEMA5A-Antibody-Immunohistochemistry-Paraffin-NBP2-20291-img0002.jpg)
![Western Blot: ICA1 Antibody [NBP2-16909] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ICA1-Antibody-Western-Blot-NBP2-16909-img0001.jpg "Western Blot: ICA1 Antibody [NBP2-16909] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ICA1 Antibody [NBP2-16909] - Immunohistochemical analysis of paraffin-embedded A2780-CP70 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ICA1-Antibody-Immunohistochemistry-Paraffin-NBP2-16909-img0002.jpg)
![Western Blot: Vimentin [p Ser56] Antibody (GT11512) [NBP3-13578] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody [GT11512] (NBP3-13578) diluted at 1:3000.](http://images.novusbio.com/fullsize/Vimentin-p-Ser56-Antibody-GT11512-Western-Blot-NBP3-13578-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vimentin [p Ser56] Antibody (GT11512) [NBP3-13578] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody [GT11512] (NBP3-13578) diluted at 1:3000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin [p Ser56] Antibody (GT11512) [NBP3-13578] - Vimentin (phospho Ser56) antibody [GT11512] detects Vimentin (phospho Ser56) protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin (phospho Ser56) antibody [GT11512] (NBP3-13578) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Vimentin-p-Ser56-Antibody-GT11512-Immunohistochemistry-Paraffin-NBP3-13578-img0002.jpg)
![Western Blot: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF/CCN2 antibody [GT28711] (NBP3-13686) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-GT28711-Western-Blot-NBP3-13686-img0001.jpg "Western Blot: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF/CCN2 antibody [GT28711] (NBP3-13686) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - CTGF/CCN2 antibody [28711] detects CTGF/CCN2 protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CTGF/CCN2 stained by CTGF/CCN2 antibody [28711] (NBP3-13686) diluted at 1:500.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-GT28711-Immunocytochemistry-Immunofluorescence-NBP3-13686-img0003.jpg)
![Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Western-Blot-NBP3-13263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1148] Antibody [NBP3-13263] - EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13263-img0002.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13265-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13265] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13265-img0002.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13228-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13228] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13228-img0002.jpg)
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-Antibody-HL1022-L858R-Mutant-Western-Blot-NBP3-13683-img0001.jpg "Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-910202420115098.jpg)
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts (30 | 15 | 15 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-91020242063323.jpg)
![Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:5000.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:5000.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:1000.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0001.jpg)
![Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0002.jpg)
![Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/PRAS40-Antibody-Western-Blot-NBP1-55261-img0004.jpg "Western Blot: PRAS40 Antibody [NBP1-55261] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate")
![Immunohistochemistry: PRAS40 Antibody [NBP1-55261] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic in endothelial cells in blood vessels Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-NBP1-55261-img0003.jpg)