| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48438 | |||
| Azide and BSA Free | NBP3-11595 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21659-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21158-25ul | |||
| Azide and BSA Free | NBP3-44315 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NB100-2658AFP405 | |||
| Alexa Fluor Plus 488 | NB100-2658AFP488 | |||
| Alexa Fluor Plus 555 | NB100-2658AFP555 | |||
| Alexa Fluor Plus 594 | NB100-2658AFP594 | |||
| Alexa Fluor Plus 647 | NB100-2658AFP647 | |||
| Alexa Fluor Plus 680 | NB100-2658AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2658-0.025mg | |||
![Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-59164-img0001.jpg "Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: OCT4 Antibody [NBP2-59164] - ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the antibody against OCT4 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/OCT4-Antibody-Chromatin-Immunoprecipitation-NBP2-59164-img0003.jpg)
![ELISA: OCT4 Antibody [NBP2-59164] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against OCT4. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100,000.](http://images.novusbio.com/fullsize/OCT4-Antibody-ELISA-NBP2-59164-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (136-4B5) [NBP2-44840] - Human Tonsil stained with CD45 antibody (136-4B5) [NBP2-44840].](http://images.novusbio.com/fullsize/CD45-Antibody-136-4B5-Immunohistochemistry-Paraffin-NBP2-44840-img0001.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (136-4B5) [NBP2-44840] - Human Tonsil stained with CD45 antibody (136-4B5) [NBP2-44840].")
![Flow Cytometry: CD45 Antibody (136-4B5) [NBP2-44840] - Flow Cytometric Analysis of live Jurkat cells unstained (grey) and CD45 MAb (136-4B5) conjugated with CF680 (purple).](http://images.novusbio.com/fullsize/CD45-Antibody-136-4B5-Flow-Cytometry-NBP2-44840-img0002.jpg)
![Immunohistochemistry: CD45 Antibody (SR1188) [NBP3-21659] - Immunohistochemical analysis of paraffin-embedded human spleen, using NBP3-21659.](http://images.novusbio.com/fullsize/nbp3-21659_rabbit-cd45-mab-sr1188-5720231524616.jpg "Immunohistochemistry: CD45 Antibody (SR1188) [NBP3-21659] - Immunohistochemical analysis of paraffin-embedded human spleen, using NBP3-21659.")
![Western Blot: CD45 Antibody (SR1188) [NBP3-21659] - Western blot analysis using NBP3-21659 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21659_rabbit-cd45-mab-sr1188-5720231552188.jpg)
![Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] -Human peripheral blood lymphocytes were stained with mouse anti- human.](http://images.novusbio.com/fullsize/antibody/nbp1-28509_mouse-monoclonal-cd45-antibody-f10-89-4-flow-cytometry-1322024115231..jpg "Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] -Human peripheral blood lymphocytes were stained with mouse anti- human.")
![Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/FKBP38-Antibody-Western-Blot-NBP1-77909-img0001.jpg "Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.")
![Immunohistochemistry: OCT4 Antibody (H7) - BSA Free [NBP3-48273] - Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using (OCT4 Antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-48273_mouse-oct4-mab-h7-bsa-free-28420251235659.jpg "Immunohistochemistry: OCT4 Antibody (H7) - BSA Free [NBP3-48273] - Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using (OCT4 Antibody) at dilution of 1:500")
![Western Blot: OCT4 Antibody (H7) - BSA Free [NBP3-48273] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (OCT4 Antibody) at dilution of 1:10000](http://images.novusbio.com/fullsize/nbp3-48273_mouse-oct4-mab-h7-bsa-free-284202512315752.jpg)