| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56472SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84499-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41250-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41103-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Analysis of anti-CNR1 / CB1 antibody with HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunocytochemistry-Immunofluorescence-NLS32-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Analysis of anti-CNR1 / CB1 antibody with HEK293 human embryonic kidney cells.")
![Immunohistochemistry-Paraffin: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Brain, substantia nigra](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunohistochemistry-Paraffin-NLS32-img0013.jpg)
![Immunohistochemistry-Paraffin: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Analysis of anti-CNR1 / CB1 antibody with brain, neuron.](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunohistochemistry-Paraffin-NLS32-img0008.jpg)
![Immunohistochemistry-Paraffin: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Analysis of anti-CNR1 / CB1 antibody with untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunohistochemistry-Paraffin-NLS32-img0010.jpg)
![Immunohistochemistry-Paraffin: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Analysis of anti-CNR1 / CB1 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunohistochemistry-Paraffin-NLS32-img0011.jpg)
![Immunohistochemistry-Paraffin: Cannabinoid R1/CB1/CNR1 Antibody [NLS32] - Anti-CNR1 / CB1 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Cannabinoid-R1-CB1-CNR1-Antibody-Immunohistochemistry-Paraffin-NLS32-img0012.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Hypothalamus.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0006.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Hypothalamus.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Anti-CHRM2 / M2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Parkinson's Disease Neuron Lewy BodyBrain, Parkinson's Disease Neuron Lewy Body.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Human lung, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval dilution 16 ug/ml.](http://images.novusbio.com/fullsize/Neuromedin-B-R-NMBR-Antibody-Immunohistochemistry-Paraffin-NLS825-img0006.jpg "Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Human lung, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval dilution 16 ug/ml.")
![Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Analysis of anti-NMBR antibody with human brain olfactory bulb.](http://images.novusbio.com/fullsize/Neuromedin-B-R-NMBR-Antibody-Immunohistochemistry-Paraffin-NLS825-img0001.jpg)
![Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Analysis of anti-NMBR antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Neuromedin-B-R-NMBR-Antibody-Immunohistochemistry-Paraffin-NLS825-img0002.jpg)
![Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Neuromedin-B-R-NMBR-Antibody-Immunohistochemistry-Paraffin-NLS825-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuromedin B R/NMBR Antibody [NLS825] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Dilution 16 ug/ml.](http://images.novusbio.com/fullsize/Neuromedin-B-R-NMBR-Antibody-Immunohistochemistry-Paraffin-NLS825-img0005.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Anti-GRM3 / MGLUR3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS899-img0005.jpg "Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Anti-GRM3 / MGLUR3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Analysis of anti-GRM3 / MGLUR3 antibody with transfection control at 16 ug/ ml.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS899-img0002.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Analysis of anti-GRM3 / MGLUR3 antibody with brain, pituitary at 16 ug/ ml.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS899-img0001.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Analysis of anti-GRM3 / MGLUR3 antibody with transfected cells expressing metabotropic Glutamate Receptor 3 at 16 ug/ ml.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS899-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS899] - Analysis of anti-GRM3 / MGLUR3 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/mGluR3-Antibody-Immunohistochemistry-Paraffin-NLS899-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: mGluR3 Antibody - BSA Free [NLS899] - Immunocytochemistry staining of untransfected HEK293 human embryonic kidney cells.](http://images.novusbio.com/fullsize/nls899_rabbit-mglur3-pab-bsa-free-109202411112810.jpg)
![Western Blot: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of neurotensin receptor 2 in the 1) absence and 2) presence of immunizing peptide in mouse brain lysate using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Neurotensin-Receptor-2-Antibody-Western-Blot-NB100-56472-img0004.jpg "Western Blot: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of neurotensin receptor 2 in the 1) absence and 2) presence of immunizing peptide in mouse brain lysate using this antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of human brain, cerebellum using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Neurotensin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB100-56472-img0003.jpg)
![Western Blot: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Analysis of CNR2 expression in transfected 293T cell line by CNR2 monoclonal antibody (M01), clone 3C7.Lane 1: CNR2 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cannabinoid-R2-CB2-CNR2-Antibody-3C7-Western-Blot-H00001269-M01-img0005.jpg "Western Blot: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Analysis of CNR2 expression in transfected 293T cell line by CNR2 monoclonal antibody (M01), clone 3C7.Lane 1: CNR2 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Analysis of monoclonal antibody to CNR2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cannabinoid-R2-CB2-CNR2-Antibody-3C7-Immunocytochemistry-Immunofluorescence-H00001269-M01-img0003.jpg)
![ELISA: Cannabinoid R2/CB2/CNR2 Antibody (3C7) [H00001269-M01] - Detection limit for recombinant GST tagged CNR2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cannabinoid-R2-CB2-CNR2-Antibody-3C7-ELISA-H00001269-M01-img0006.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-212024939131.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249432857.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249342722.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251832019.jpg)
![<b>Genetic Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518404315.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518442215.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518423013.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518412910.jpg)
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518335919.jpg)
![<b>Independent Antibodies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251838289.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518404314.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251840545.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-141120251838173.jpg)
![Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584713.jpg "Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.")
![ELISA: Glucagon Antibody [NBP3-23954] - ELISA Results of Glucagon Antibody. Each well was coated with 1µg of conjugate. The starting concentration of antibody in the dilution series was 5 µg/ml. The titer is 1:34,800 Glucagon - Free peptide [Green Line], 1:47200 Glucagon Standard - BSA conjugated [Blue Line], and 1:20,500 Glucagon - BSA conjugated [Purple Line]. Each point on the Y-axis represents a 3-fold dilution. 3% Fish Gel, HRP conjugated Goat anti-Rabbit IgG (H&L), and TMB substrate were used for detection.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-23120252358477.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 100% Methanol. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-2312025235983.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: U20S cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-23120252358471.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 4% PFA. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584747.jpg)
![Western Blot: Glucagon Antibody [NBP3-23954] - Western Blot of Glucagon Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: COS-7 Lysate - reduced (20µg). Lane 3: BSA Conjugated Glucagon peptide - reduced (0.02µg).Lane 4: Insulin - reduced (0.05µg). Primary Antibody: Anti-Glucagon [Rabbit] Antibody at 1.0µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (MX10) Peroxidase conjugated at 1:70,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 1hr at RT.Expected MW: ~21kDa. Observed MW: endogenous detection in COS-7 Lysate at ~35kDa. Glucagon peptide is detected at the MW of BSA. No cross-reactivity with insulin is observed.Exposure: 25 sec.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584717.jpg)
![Immunohistochemistry: Glucagon Antibody [NBP3-23954] - Immunohistochemistry results using Glucagon Antibody. Tissue: alpha cells in CD1 mouse pancreatic islets. Fixation: 4% paraformaldehyde. Antigen Retrieval: 10mM Sodium Citrate buffer for 10 mins at 95-100°C. Blocking: PBS, 1% ovalbumin, 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 1:100 overnight at RT. Secondary Antibody: Anti-Rabbit Alexa Fluor 488 at 1:500 for 1hr at RT. Original magnification 20x. Courtesy of Prof. Merighi, University of Turin.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584723.jpg)
![Immunocytochemistry/Immunofluorescence: ZDHHC9 Antibody [NBP1-84499] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize/ZDHHC9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84499-img0006.jpg "Immunocytochemistry/Immunofluorescence: ZDHHC9 Antibody [NBP1-84499] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: ZDHHC9 Antibody [NBP1-84499] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ZDHHC9-Antibody-Immunohistochemistry-Paraffin-NBP1-84499-img0001.jpg)
![OPN1MW2 antibody [HL3435] detects OPN1MW2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: OPN1MW2 stained by OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunocytochemistry-immunofluorescence-1411202518425216.jpg "OPN1MW2 antibody [HL3435] detects OPN1MW2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: OPN1MW2 stained by OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![OPN1MW2 antibody [HL3435] detects OPN1MW2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: OPN1MW2 stained by OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunohistochemistry-paraffin-141120251843139.jpg)
![OPN1MW2 antibody [HL3435] detects OPN1MW2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: OPN1MW2 stained by OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunohistochemistry-paraffin-14112025184339.jpg)
![Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-western-blot-141120251826013.jpg)
![OPN1MW2 antibody [HL3435] detects OPN1MW2 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: OPN1MW2 stained by OPN1MW2 antibody [HL3435] (NBP3-48812) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-30883_rabbit-polyclonal-stathmin-1-antibody-immunohistochemistry-frozen-141120251855410.jpg)
![Western Blot: CXCR3 Antibody [NBP2-41250] - Analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NBP2-41250-img0001.jpg "Western Blot: CXCR3 Antibody [NBP2-41250] - Analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NBP2-41250] - Immunocytochemistry of CXCR3 in HepG2 cells with CXCR3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41250-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody - BSA Free [NBP2-41250] - Immunofluorescence of CXCR3 in HepG2 cells with CXCR3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41250_rabbit-polyclonal-cxcr3-antibody-2711202420414587.jpg)
![Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Western-Blot-NBP2-41103-img0001.jpg "Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry-Paraffin: Melanocortin-4 R Antibody [NBP2-41103] - human brain tissue with MC4R antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunohistochemistry-Paraffin-NBP2-41103-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-4 R Antibody [NBP2-41103] - Human Brain cells with MC4R antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunofluorescence-NBP2-41103-img0003.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-271120242022912.jpg)
![Immunocytochemistry/ Immunofluorescence: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunofluorescence of Melanocortin-4 R in Human Brain cells with Melanocortin-4 R antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419562138.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419531688.jpg)