| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03456-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97782-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00079-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34563-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26386-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26494-50ul | |||
![Immunohistochemistry:[NBP3-29341]-Detection of human HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1.](http://images.novusbio.com/fullsize/nbp3-29341_rabbit-hmgn1-hmg14-pab-23120251225695.jpg "Immunohistochemistry:[NBP3-29341]-Detection of human HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1.")
![Western Blot: XRCC4 Antibody [NBP3-03456] - Analysis of extracts from normal (control) and xRCC4 knockout (KO) HeLa cells, using xRCC4 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/XRCC4-Antibody-Knockout-Validated-NBP3-03456-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: XRCC4 Antibody [NBP3-03456] - Analysis of extracts from normal (control) and xRCC4 knockout (KO) HeLa cells, using xRCC4 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/RPA14-Antibody-Western-Blot-NBP2-97782-img0004.jpg "Western Blot: RPA14 Antibody [NBP2-97782] - Anti-RPA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: HL-60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa")
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0003.jpg)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP2-97782] - Immunochemical staining of human RPA3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP2-97782-img0002.jpg)
![Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Western-Blot-NBP3-16286-img0001.jpg "Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug Rad50 antibody (NBP3-16286). Western blot was performed from the immunoprecipitate using Rad50 antibody (NBP3-16286) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Immunoprecipitation-NBP3-16286-img0002.jpg)
![Western Blot: p53R2 Antibody [NBP3-00079] - Anti-p53R2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP3-00079-img0002.jpg "Western Blot: p53R2 Antibody [NBP3-00079] - Anti-p53R2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP3-00079] - Immunochemical staining of human RRM2B in human prostatic carcinomal with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP3-00079-img0003.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP3-00079] - Immunochemical staining of human p53R2 in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP3-00079-img0004.jpg)
![Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Western-Blot-NBP3-16353-img0002.jpg "Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353] - Immunohistochemistry of paraffin-embedded human colon using Chk1 Rabbit mAb (NBP3-16353) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Immunohistochemistry-Paraffin-NBP3-16353-img0001.jpg)
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0005.jpg "Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).")
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0004.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44980-img0001.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - IHC-Prediluted [NBP2-44980] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202411351620.jpg "Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate). All lanes: p53 [p Thr55] Antibody at 1.28ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 KDa Observed band size: 53 KDa")
![Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Staining of 293 cells (treated with 50mM Calyculin A for 30min) with p53 [p Thr55] Antibody (3C3) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202412493626.jpg)
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-262202415355749.jpg "Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-27220248313715.jpg)
![Flow Cytometry: ATM Antibody (3G11) [NBP3-26494] - Overlay histogram showing Hela cells stained with ATM Antibody (3G11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-282202414312732.jpg)