| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02946-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26044 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-173SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-230SS | |||
![Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Western-Blot-NBP3-02946-img0004.jpg "Western Blot: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of extracts of various cell lines, using HMGN1/HMG14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Immunocytochemistry/Immunofluorescence: HMGN1/HMG14 Antibody [NBP3-02946] - Analysis of HeLa cells using HMGN1/HMG14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGN1-HMG14-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02946-img0001.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Immunohistochemistry: Wee1 Antibody - BSA Free [NBP3-47479] - Immunohistochemistry of paraffin-embedded rat testis tissue slide using (Wee1 Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-47479_rabbit-wee1-pab-bsa-free-28420251385042.jpg "Immunohistochemistry: Wee1 Antibody - BSA Free [NBP3-47479] - Immunohistochemistry of paraffin-embedded rat testis tissue slide using (Wee1 Antibody) at dilution of 1:200")
![Western Blot: Wee1 Antibody - BSA Free [NBP3-47479] - Jurkat cells were subjected to SDS PAGE followed by western blot with ( Wee1 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47479_rabbit-wee1-pab-bsa-free-284202512593043.jpg)
![Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-278202416285244.jpg "Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MLH1 Antibody (PD01-61) [NBP3-32599] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-298202413585516.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-RM214-Western-Blot-NBP3-18534-img0001.jpg "Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-18534] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-18534_rabbit-histone-h2ax-mab-rm214-immunocytochemistry-immunofluorescence-189202523403513.png)
![Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023] - Paraffin-embedded lung CA patient tumor, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RPA14-Antibody-Immunohistochemistry-Paraffin-NBP1-32023-img0003.jpg "Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023] - Paraffin-embedded lung CA patient tumor, using antibody at 1:100 dilution.")
![Western Blot: RPA14 Antibody [NBP1-32023] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPA14 antibody (NBP1-32023) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32023_rabbit-polyclonal-rpa14-antibody-9102024205694.jpg)
![Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser33]-Antibody-Western-Blot-NBP3-13309-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NB100-173-img0001.jpg "Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500")
![Western Blot: PUMA Antibody [NB500-230] - WB detection of PUMA in transfected U20S cells using NB500-230 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB500-230-img0002.jpg "Western Blot: PUMA Antibody [NB500-230] - WB detection of PUMA in transfected U20S cells using NB500-230 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target.")
![Western Blot: Kv10.2 Antibody [NBP2-83132] - WB Suggested Anti-KCNH5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/Kv10.2-Antibody-Western-Blot-NBP2-83132-img0002.jpg "Western Blot: Kv10.2 Antibody [NBP2-83132] - WB Suggested Anti-KCNH5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate")
![Immunohistochemistry: Kv10.2 Antibody [NBP2-83132] - Human Brain](http://images.novusbio.com/fullsize/Kv10.2-Antibody-Immunohistochemistry-NBP2-83132-img0001.jpg)
![Immunohistochemistry:[NBP3-29341]-Detection of human HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1.](http://images.novusbio.com/fullsize/nbp3-29341_rabbit-hmgn1-hmg14-pab-23120251225695.jpg "Immunohistochemistry:[NBP3-29341]-Detection of human HMGN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1.")