| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52549-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98634-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56359SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52726SS | |||
| Azide and BSA Free | NBP2-80943 | |||
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0004.jpg "Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)")
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded esophageal cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0003.jpg)
![Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Flow-Cytometry-NBP2-52549-img0001.jpg)
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against HEK293 (1) and RAD23A (AA: 1-363)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded bladder cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0002.jpg)
![ELISA: Rad23 Antibody (3E3F4) [NBP2-52549] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Rad23-Antibody-3E3F4-ELISA-NBP2-52549-img0006.jpg)
![Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Western-Blot-NBP2-73783-img0002.jpg "Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.")
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0006.jpg)
![Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Western-Blot-NBP2-73783-img0001.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0003.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0004.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0005.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0007.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: DMAP1 Antibody (JE55-31) [NBP3-32271] - Western blot analysis of DMAP1 on different lysates with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: F9 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32271) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-278202416285275.jpg "Western Blot: DMAP1 Antibody (JE55-31) [NBP3-32271] - Western blot analysis of DMAP1 on different lysates with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: F9 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32271) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32271) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-298202414262626.jpg)
![Immunocytochemistry/ Immunofluorescence: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunocytochemistry analysis of HeLa cells labeling DMAP1 with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-292024119235.jpg)
![Flow Cytometry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Flow cytometric analysis of HeLa cells labeling DMAP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32271, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-392024884644.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: hnRNP C1 + C2 Antibody [NBP2-98634] - Anti-hnRNP C1 + C2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Western-Blot-NBP2-98634-img0006.jpg "Western Blot: hnRNP C1 + C2 Antibody [NBP2-98634] - Anti-hnRNP C1 + C2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunochemical staining of human hnRNP C1 + C2 in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-98634-img0005.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunochemical staining of human hnRNP C1 + C2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-98634-img0004.jpg)
![Immunoprecipitation: hnRNP C1 + C2 Antibody [NBP2-98634] - hnRNP C1 + C2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-hnRNP C1 + C2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-hnRNP C1 + C2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunoprecipitation-NBP2-98634-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunofluorescence staining of hnRNP C1 + C2 in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98634 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-98634_rabbit-polyclonal-hnrnp-c1-c2-antibody-23520251129398.png)
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: HCT 116 cell lysate (20 ug/Lane) Lane 6: THP-1 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Human kidney tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-288202412461611.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: HCT 116 cell lysate (20 ug/Lane) Lane 6: THP-1 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Human kidney tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32918) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-29820241492655.jpg)
![Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Immunocytochemistry analysis of HeLa cells labeling Ribosomal Protein S6/RPS6 with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32918) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-29202410234256.jpg)
![Flow Cytometry: Ribosomal Protein S6/RPS6 Antibody (A6B9-R) [NBP3-32918] - Flow cytometric analysis of HeLa cells labeling Ribosomal Protein S6/RPS6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32918, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32918_mouse-ribosomal-protein-s6-rps6-mab-a6b9-r-392024884653.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Western-Blot-NBP2-54545-img0005.jpg "Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).")
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0002.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Protein-Array-NBP2-54545-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33083-img0004.jpg "Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0008.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP1-33083-img0006.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0005.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33083_rabbit-polyclonal-rfc4-antibody-910202420134095.jpg)
![Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-56359-img0005.jpg "Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.")
![Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NB100-56359-img0004.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Immunohistochemistry-Paraffin-NBP2-52726-img0002.jpg "Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.")
![Flow (Intracellular): Senataxin Antibody (4G1) [NBP2-52726] - An intracellular stain was performed on HepG2 cells with Senataxin Antibody (4G1) antibody NBP2-52726 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Flow-Intracellular-NBP2-52726-img0001.jpg)