| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20097-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20095-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
![Western Blot: PNK Antibody (JE64-21) [NBP3-32835] - Western blot analysis of PNK on different lysates with Rabbit anti-PNK antibody (NBP3-32835) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32835) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-288202412442958.jpg "Western Blot: PNK Antibody (JE64-21) [NBP3-32835] - Western blot analysis of PNK on different lysates with Rabbit anti-PNK antibody (NBP3-32835) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32835) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PNK Antibody (JE64-21) [NBP3-32835] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PNK antibody (NBP3-32835) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32835) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-298202414333612.jpg)
![Immunocytochemistry/ Immunofluorescence: PNK Antibody (JE64-21) [NBP3-32835] - Immunocytochemistry analysis of Hela cells labeling PNK with Rabbit anti-PNK antibody (NBP3-32835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PNK antibody (NBP3-32835) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32835_rabbit-pnk-mab-je64-21-2920241192375.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Human Lung, Non-Small Cell Carcinoma. formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PAK6-Antibody-Immunohistochemistry-Paraffin-NLS6942-img0003.jpg "Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Human Lung, Non-Small Cell Carcinoma. formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PAK6 Antibody [NLS6942] - Analysis of anti-PAK6 antibody with human brain, neurons and glia at 3.5 ug/ml.](http://images.novusbio.com/fullsize/PAK6-Antibody-Immunohistochemistry-Paraffin-NLS6942-img0002.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0003.jpg "Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with testis at 5.2 ug/ml.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-NLS7979-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0002.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-Methylated-DNA-Immunoprecipitation-NBP2-59178-img0002.jpg "Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).")
![ELISA: 5-hmC Antibody (C.15220001) [NBP2-59178] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-ELISA-NBP2-59178-img0001.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - (A): Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hmC Antibody (C.15220001) followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0004.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 ug/ml (dilution 1:500) of the 5-hmC Antibody (C.15220001), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-2742023924237.png "Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-28B-8 (NBP3-20097) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202392428.png)
![Flow Cytometry: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28B-8 (NBP3-20097, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202396466.png)
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-274202392427.png "Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (NBP3-20095, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924282.png)
![Western Blot: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Human colon tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (NBP3-20095) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924210.png)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48351-img0003.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).")
![Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0059.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-249-img0061.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0002.jpg)
![Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Peptide-Chromatin-Immunoprecipitation-NB21-2089PEP-img0002.jpg "Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide")