| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56489SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54594-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32011-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08957 | |||
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: hnRNP A1 Antibody [NBP1-32161] - Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody [NBP1-32161] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32161-img0002.jpg)
![Immunohistochemistry-Paraffin: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-32161-img0008.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0004.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample(30 ug of whole cell lysate) A:H1299 B:HeLa S3 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0005.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - A. 30 ug PC-12 whole cell lysate/extract.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0007.jpg)
![Immunohistochemistry: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody (NBP1-32161) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-32161_rabbit-polyclonal-hnrnp-a1-antibody-9102024205646.jpg)
![Western Blot: Importin-9 Antibody [NB100-56489] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Importin-9-Antibody-Knockout-Validated-NB100-56489-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Importin-9 Antibody [NB100-56489] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Importin-9 Antibody [NB100-56489] - Analysis of Importin-9 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Importin-9-Antibody-Western-Blot-NB100-56489-img0003.jpg)
![Immunohistochemistry-Paraffin: Importin-9 Antibody [NB100-56489] - Analysis of human brain, cortex using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Importin-9-Antibody-Immunohistochemistry-Paraffin-NB100-56489-img0002.jpg)
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Western-Blot-NBP2-15723-img0009.jpg "Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.")
![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15723-img0007.jpg)
![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15723-img0008.jpg)
![Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723] - IP samples: HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of CTNNA1 protein by 4 ug alpha 1 Catenin antibody [N1N3] 5 % SDS-PAGE The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunoprecipitation-NBP2-15723-img0006.jpg)
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/antibody/nbp2-15723_rabbit-polyclonal-catenin-alpha-1-antibody-western-blot-662023103211..jpg)
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate), A: 293T, B: A431, C: HeLa 5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/antibody/nbp2-15723_rabbit-polyclonal-catenin-alpha-1-antibody-western-blot-662023103415..jpg)
![alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Green: alpha Catenin antibody [N1N3] (NBP2-15723) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15723_rabbit-polyclonal-catenin-alpha-1-antibody-immunohistochemistry-paraffin-2611202519575.jpg)
![Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot Analysis of human A549 cell lysate using SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Western-Blot-NBP2-53407-img0009.jpg "Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot Analysis of human A549 cell lysate using SOX2 Antibody (SOX2/1791).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0006.jpg)
![Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Western-Blot-NBP2-53407-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX2 Antibody (SOX2/1791).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Immunohistochemistry-Paraffin-NBP2-53407-img0005.jpg)
![Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1791) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1791-Protein-Array-NBP2-53407-img0007.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-30888-img0008.jpg "Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30888-img0007.jpg)
![Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - A549 xenograft, using SET antibody, isoform 2 specific at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SET-Antibody-Immunohistochemistry-Paraffin-NBP1-30888-img0003.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NB100-93304-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-310202416284928.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-31020241628498.jpg)
![Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0007.jpg "Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP2-16337] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16337-img0004.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16337-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP2-16337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0005.jpg)
![Western Blot: Enolase 1 Antibody [NBP2-16337] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0006.jpg)
![Immunoprecipitation: Enolase 1 Antibody [NBP2-16337] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of ENO1 protein by 2. 5 ug of ENO1 antibody 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunoprecipitation-NBP2-16337-img0003.jpg)
![Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MSH2-Antibody-Western-Blot-NB100-621-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunohistochemistry-Paraffin-NB100-621-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MSH2 Antibody [NB100-621] - Detection of human and mouse MSH2 by Western blot and immunoprecipitation using NB100-621. Detection: ECL with a 3 minute exposure.](http://images.novusbio.com/fullsize/MSH2-Antibody-Western-Blot-NB100-621-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunohistochemistry-Paraffin-NB100-621-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MSH2 Antibody [NB100-621] - Detection of human MSH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for IP at 6 ug per reaction. MSH2 was also immunoprecipitated by another rabbit anti-MSH2 antibody. For blotting immunoprecipitated MSH2, NB100-621 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunoprecipitation-NB100-621-img0009.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0014.jpg "Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of monoclonal antibody to RPS5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Immunocytochemistry-Immunofluorescence-H00006193-M02-img0008.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3 Analysis of RPS5 expression in HeLa.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0010.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in PC-12.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0011.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in Raw 264.7.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0012.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in NIH/3T3.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0013.jpg)
![ELISA: RPS5 Antibody (4H3) [H00006193-M02] - Detection limit for recombinant GST tagged RPS5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-ELISA-H00006193-M02-img0009.jpg)
![Western Blot: RCN2 Antibody [NBP2-32011] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/RCN2-Antibody-Western-Blot-NBP2-32011-img0008.jpg "Western Blot: RCN2 Antibody [NBP2-32011] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: RCN2 Antibody [NBP2-32011] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32011-img0007.jpg)
![Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0015.jpg)
![Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0009.jpg)
![Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0010.jpg)
![Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining in human epididymis and pancreas tissues using anti-RCN2 antibody. Corresponding RCN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human cerebral cortex, epididymis, pancreas and testis using Anti-RCN2 antibody NBP2-32011 (A) shows similar protein distribution across tissues to independent antibody NBP2-32010 (B).](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0013.jpg)
![Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32011] - Staining of human testis.](http://images.novusbio.com/fullsize/RCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-32011-img0014.jpg)
![Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Western-Blot-NBP3-07211-img0007.jpg "Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).")
![Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunocytochemistry-Immunofluorescence-NBP3-07211-img0002.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0006.jpg)
![Flow Cytometry: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Flow-Cytometry-NBP3-07211-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0003.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0004.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0005.jpg)