| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60241-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03161-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35411-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38633-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59442-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94554-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59468-50ul | |||
![Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Western-Blot-NBP2-60241-img0001.jpg "Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NBP2-60241-img0002.jpg)
![Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-27910_mouse-wdr77-mab-8b2f7-271120241918625.jpg "Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-03161_rabbit-polyclonal-peroxiredoxin-3-antibody-5220251639631.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.")
![Western Blot: Catenin alpha 1 Antibody (9O1V3) [NBP3-15613] - Western blot analysis of extracts of various cell lines, using Catenin alpha 1 antibody (NBP3-15613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-9O1V3-Western-Blot-NBP3-15613-img0002.jpg "Western Blot: Catenin alpha 1 Antibody (9O1V3) [NBP3-15613] - Western blot analysis of extracts of various cell lines, using Catenin alpha 1 antibody (NBP3-15613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Catenin alpha 1 Antibody (9O1V3) [NBP3-15613] - Western blot analysis of extracts from normal (control) and Catenin alpha 1 knockout (KO) 293T cells, using Catenin alpha 1 antibody (NBP3-15613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-9O1V3-Knockout-Validated-NBP3-15613-img0001.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0001.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of PC-12 cells, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0002.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Biological Strategies Validation. </b>Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411334047.jpg "Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunoprecipitation: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug Proteasome 20S alpha 5 antibody. Western blot was performed from the immunoprecipitate using Proteasome 20S alpha 5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411404039.jpg)
![Western Blot: eIF3K Antibody [NBP3-38633] - Western blot analysis of various lysates using eIF3K Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38633_rabbit-eif3k-pab-9122024151952.jpg "Western Blot: eIF3K Antibody [NBP3-38633] - Western blot analysis of various lysates using eIF3K Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates](http://images.novusbio.com/fullsize/Fumarase-Antibody-3F6-Western-Blot-NBP2-59442-img0001.jpg "Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates")
![Western Blot: NME4 Antibody [NBP2-94554] - Analysis of extracts of various cell lines, using NME4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/NME4-Antibody-Western-Blot-NBP2-94554-img0003.jpg "Western Blot: NME4 Antibody [NBP2-94554] - Analysis of extracts of various cell lines, using NME4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Western-Blot-NBP2-59468-img0001.jpg "Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Analysis of PA2G4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of PA2G4 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-59468-img0002.jpg)
![Western Blot: p57 Kip2 Antibody [NBP3-27877] - Western blot analysis of p57 Kip2 in 293 cell lysate with p57 Kip2 antibody at (A) 1 and (B) 2 u/mL.](http://images.novusbio.com/fullsize/nbp3-27877_rabbit-p57-kip2-pab-2711202419313278.gif "Western Blot: p57 Kip2 Antibody [NBP3-27877] - Western blot analysis of p57 Kip2 in 293 cell lysate with p57 Kip2 antibody at (A) 1 and (B) 2 u/mL.")
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody [NBP3-27877] - Immunofluorescence of p57 Kip2 in 293 cells with p57 Kip2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp3-27877_rabbit-p57-kip2-pab-2711202420432382.jpg)