| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35743-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37984-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60241-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03161-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59456-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93065-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35411-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59425-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59399-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59461-50ul | |||
![Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35743_rabbit-hmgb2-pab-912202415171171.jpg "Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: DLG7/HURP Antibody [NBP3-37984] - Western blot analysis of lysates from K562 cells, using DLG7/HURP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-37984_rabbit-dlg7-hurp-pab-10122024124391.jpg "Western Blot: DLG7/HURP Antibody [NBP3-37984] - Western blot analysis of lysates from K562 cells, using DLG7/HURP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: DLG7/HURP Antibody [NBP3-37984] - Immunofluorescence analysis of U2OS cells using DLG7/HURP Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37984_rabbit-dlg7-hurp-pab-1012202411572691.jpg)
![Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Western-Blot-NBP2-60241-img0001.jpg "Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NBP2-60241-img0002.jpg)
![Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-03161_rabbit-polyclonal-peroxiredoxin-3-antibody-5220251639631.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.")
![Western Blot: RUVBL1 Antibody (1D6) [NBP2-59456] - Lane 1: Raji cell lysates](http://images.novusbio.com/fullsize/RUVBL1-Antibody-1D6-Western-Blot-NBP2-59456-img0001.jpg "Western Blot: RUVBL1 Antibody (1D6) [NBP2-59456] - Lane 1: Raji cell lysates")
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody (1D6) [NBP2-59456] - Analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/RUVBL1-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-59456-img0002.jpg)
![Western Blot: SDHD Antibody [NBP2-93065] - Analysis of extracts of various cell lines, using SDHD at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/SDHD-Antibody-Western-Blot-NBP2-93065-img0001.jpg "Western Blot: SDHD Antibody [NBP2-93065] - Analysis of extracts of various cell lines, using SDHD at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411334047.jpg "Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunoprecipitation: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug Proteasome 20S alpha 5 antibody. Western blot was performed from the immunoprecipitate using Proteasome 20S alpha 5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411404039.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Biological Strategies Validation. </b>Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0001.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of various cell lines, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: MgcRacGAP/RACGAP1 Antibody (10F7U6) [NBP3-15700] - Western blot analysis of extracts of PC-12 cells, using MgcRacGAP/RACGAP1 antibody (NBP3-15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-10F7U6-Western-Blot-NBP3-15700-img0002.jpg)
![Western Blot: Peroxiredoxin 3 Antibody (1F8) [NBP2-59425] - Lane 1: Recombinant protein, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: TF1 cell lysates, Lane 5: U87MG cell lysates, Lane 6: Raji cell lysates, Lane 7: 293T cell lysates, Lane 8: Jurkat cell lysates, Lane 9: MCF7 cell lysates](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-1F8-Western-Blot-NBP2-59425-img0001.jpg "Western Blot: Peroxiredoxin 3 Antibody (1F8) [NBP2-59425] - Lane 1: Recombinant protein, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: TF1 cell lysates, Lane 5: U87MG cell lysates, Lane 6: Raji cell lysates, Lane 7: 293T cell lysates, Lane 8: Jurkat cell lysates, Lane 9: MCF7 cell lysates")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (1F8) [NBP2-59425] - Analysis of Peroxiredoxin3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Peroxiredoxin3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-59425-img0002.jpg)
![Western Blot: LSM5 Antibody (25F8) [NBP2-59399] - Lane 1: HeLa cell lysates, Lane 2: K562 cell lysates, Lane 3: Jurkat cell lysates](http://images.novusbio.com/fullsize/LSM5-Antibody-25F8-Western-Blot-NBP2-59399-img0001.jpg "Western Blot: LSM5 Antibody (25F8) [NBP2-59399] - Lane 1: HeLa cell lysates, Lane 2: K562 cell lysates, Lane 3: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: LSM5 Antibody (25F8) [NBP2-59399] - Analysis of LSM5 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LSM5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/LSM5-Antibody-25F8-Immunocytochemistry-Immunofluorescence-NBP2-59399-img0002.jpg)
![Western Blot: UDP glucose dehydrogenase Antibody (2G11) [NBP2-59461] - Lane 1: HeLa cell lysates, Lane 2: NIH-3T3 cell lysates, Lane 3: HepG2 cell lysates, Lane 4: A549 cell lysates, Lane 5: MCF7 cell lysates](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-2G11-Western-Blot-NBP2-59461-img0001.jpg "Western Blot: UDP glucose dehydrogenase Antibody (2G11) [NBP2-59461] - Lane 1: HeLa cell lysates, Lane 2: NIH-3T3 cell lysates, Lane 3: HepG2 cell lysates, Lane 4: A549 cell lysates, Lane 5: MCF7 cell lysates")
![Immunocytochemistry/Immunofluorescence: UDP glucose dehydrogenase Antibody (2G11) [NBP2-59461] - Analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NBP2-59461-img0002.jpg)