| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03955SS | |||
| Azide and BSA Free | NBP2-80917 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90211-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70129 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74318 | |||
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Western-Blot-NBP2-37626-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunocytochemistry-Immunofluorescence-NBP2-37626-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-Paraffin-NBP2-37626-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-NBP2-37626-img0004.jpg)
![ELISA: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-ELISA-NBP2-37626-img0001.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0003.jpg "Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP2-37563-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0013.jpg)
![Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Flow-Cytometry-NBP2-37563-img0005.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0010.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0012.jpg)
![ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-ELISA-NBP2-37563-img0001.jpg)
![Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Western-Blot-NBP2-03955-img0013.jpg "Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the PE conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse F9 tumor cell line probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). Cell surface flow kit was used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0010.jpg)
![Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Western-Blot-NBP2-03955-img0012.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-03955 and APC-conjugated secondary antibody were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0004.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0008.jpg)
![Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-pmab-1-Flow-Cytometry-NBP2-03955-img0009.jpg)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in human ovarian cancer.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0006.jpg "Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in human ovarian cancer.")
![Immunocytochemistry/Immunofluorescence: STI1 Antibody (2E11) [H00010963-M35] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Immunocytochemistry-Immunofluorescence-H00010963-M35-img0002.jpg)
![Immunohistochemistry-Paraffin: STI1 Antibody (2E11) [H00010963-M35] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. Antibody concentration 0.75 ug/ml](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Immunohistochemistry-Paraffin-H00010963-M35-img0003.jpg)
![Flow Cytometry: STI1 Antibody (2E11) [H00010963-M35] - Analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Flow-Cytometry-H00010963-M35-img0001.jpg)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in MCF-7.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0005.jpg)
![Western Blot: STI1 Antibody (2E11) [H00010963-M35] - Analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M35), clone 2E11. Lane 1: STIP1 transfected lysate (Predicted MW: 62.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Western-Blot-H00010963-M35-img0007.jpg)
![Sandwich ELISA: STI1 Antibody (2E11) [H00010963-M35] - Detection limit for recombinant GST tagged STIP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/STI1-Antibody-2E11-Sandwich-ELISA-H00010963-M35-img0004.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0008.jpg "Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunocytochemistry-Immunofluorescence-NBP2-67405-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0011.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining in human placenta and liver tissues . Corresponding PDPN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Immunohistochemistry-Paraffin-NBP1-90211-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining in human placenta and liver tissues . Corresponding PDPN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Podoplanin Antibody [NBP1-90211] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Western-Blot-NBP1-90211-img0018.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human endometrium shows moderate membranous positivity in endothelium of lymphatic vessels.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Immunohistochemistry-Paraffin-NBP1-90211-img0011.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Immunohistochemistry-Paraffin-NBP1-90211-img0012.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human placenta shows moderate membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Immunohistochemistry-Paraffin-NBP1-90211-img0013.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human rectum shows moderate membranous positivity in endothelium of lymphatic vessels.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-Immunohistochemistry-Paraffin-NBP1-90211-img0014.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-22320239226.jpg)
![Western Blot: Glyoxalase I Antibody [NBP1-31466] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP1-31466-img0022.jpg "Western Blot: Glyoxalase I Antibody [NBP1-31466] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody [NBP1-31466] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31466-img0014.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody [NBP1-31466] - Human ovarian cancer. Glyoxalase I stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-31466-img0020.jpg)
![Western Blot: Glyoxalase I Antibody [NBP1-31466] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP1-31466-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glyoxalase I Antibody [NBP1-31466] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31466_rabbit-polyclonal-glyoxalase-i-antibody-1610202419405751.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16842] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16842-img0007.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16842] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16842-img0005.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0013.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0015.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
![Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Western-Blot-NBP2-00827-img0012.jpg "Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK3L1.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NBP2-00827-img0011.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human liver tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0010.jpg)
![Flow Cytometry: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3L1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Flow-Cytometry-NBP2-00827-img0001.jpg)
![Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3L1.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Western-Blot-NBP2-00827-img0002.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0003.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0004.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0005.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0006.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human colon tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0007.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0008.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human Kidney tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0009.jpg)
![Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Spermine synthase.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Western-Blot-NBP2-00900-img0021.jpg "Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Spermine synthase.")
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human tonsil using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0020.jpg)
![Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Spermine synthase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Flow-Cytometry-NBP2-00900-img0013.jpg)
![Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Spermine synthase monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Western-Blot-NBP2-00900-img0011.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0014.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0015.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0016.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0017.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human Kidney tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0018.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human lymphoma tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0019.jpg)
![Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of Hela cells, using anti-Spermine synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Flow-Cytometry-NBP2-00900-img0012.jpg)