| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-98931-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48197 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24557-100ul | |||
| BSA Free | NBP3-24557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61620 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18561-25ul | |||
| Azide and BSA Free | NBP3-44168 | |||
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0029.jpg "Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - Rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG at a concentration of 10 ug/ml.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0016.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0018.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0021.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0027.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0030.jpg)
![Western Blot: HCC1 Antibody (4G8) [H00009584-M01] - RNPC2 monoclonal antibody (M01), clone 4G8. Analysis of RNPC2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Western-Blot-H00009584-M01-img0012.jpg "Western Blot: HCC1 Antibody (4G8) [H00009584-M01] - RNPC2 monoclonal antibody (M01), clone 4G8. Analysis of RNPC2 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: HCC1 Antibody (4G8) [H00009584-M01] - Analysis of monoclonal antibody to RNPC2 on HeLa cell. Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Immunocytochemistry-Immunofluorescence-H00009584-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: HCC1 Antibody (4G8) [H00009584-M01] - Analysis of monoclonal antibody to RNPC2 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Immunohistochemistry-Paraffin-H00009584-M01-img0009.jpg)
![Western Blot: HCC1 Antibody (4G8) [H00009584-M01] - RNPC2 monoclonal antibody (M01), clone 4G8 Analysis of RNPC2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Western-Blot-H00009584-M01-img0011.jpg)
![Western Blot: HCC1 Antibody (4G8) [H00009584-M01] - Analysis of RBM39 expression in transfected 293T cell line by RNPC2 monoclonal antibody (M01), clone 4G8.Lane 1: RBM39 transfected lysate(58.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Western-Blot-H00009584-M01-img0013.jpg)
![ELISA: HCC1 Antibody (4G8) [H00009584-M01] - Detection limit for recombinant GST tagged RNPC2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCC1-Antibody-4G8-Sandwich-ELISA-H00009584-M01-img0010.jpg)
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM583) - Azide and BSA Free [NBP2-34433] - Formalin-fixed, paraffin-embedded colon (10X) stained with Cytokeratin, pan Antibody (SPM583).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM583-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34433-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM583) - Azide and BSA Free [NBP2-34433] - Formalin-fixed, paraffin-embedded colon (10X) stained with Cytokeratin, pan Antibody (SPM583).")
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/nbp3-43847_mouse-cox4-mab-cl3515-azide-and-bsa-free-282202512104221.jpg "Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-p-Ser1943-Antibody-GT144-Western-Blot-NBP3-13579-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human mammary gland. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-p-Ser1943-Antibody-GT144-Immunohistochemistry-Paraffin-NBP3-13579-img0004.jpg)
![Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:20000.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-p-Ser1943-Antibody-GT144-Western-Blot-NBP3-13579-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-p-Ser1943-Antibody-GT144-Western-Blot-NBP3-13579-img0002.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human colon. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-p-Ser1943-Antibody-GT144-Immunohistochemistry-Paraffin-NBP3-13579-img0003.jpg)
![MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MYH9 (phospho Ser1943) protein stained by MYH9 (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp3-13579_mouse-non-muscle-myosin-iia-p-ser-1943-mab-gt144-immunocytochemistry-immunofluorescence-2611202518213311.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0006.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Analysis in human cerebral cortex and liver tissues using NBP3-18561 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0001.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0002.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0003.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0004.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12838) [NBP3-18561] - Staining of mouse brain shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12838-Immunohistochemistry-Paraffin-NBP3-18561-img0007.jpg)
![Western Blot: Paxillin Antibody (GT7612) [NBP3-13563] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:1000.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT7612-Western-Blot-NBP3-13563-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Paxillin Antibody (GT7612) [NBP3-13563] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at focal adhesion sites by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT7612-Immunocytochemistry-Immunofluorescence-NBP3-13563-img0002.jpg)
![Immunohistochemistry-Paraffin: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Paxillin stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT7612-Immunohistochemistry-Paraffin-NBP3-13563-img0004.jpg)
![Western Blot: Paxillin Antibody (GT7612) [NBP3-13563] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:1000.](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT7612-Western-Blot-NBP3-13563-img0001.jpg)
![Immunohistochemistry-Paraffin: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Paxillin stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Paxillin-Antibody-GT7612-Immunohistochemistry-Paraffin-NBP3-13563-img0003.jpg)
![Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-GT712-Western-Blot-NBP3-13552-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin (phospho Tyr118) protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (phospho Tyr118) stained by Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-GT712-Immunocytochemistry-Immunofluorescence-NBP3-13552-img0004.jpg)
![Immunohistochemistry-Paraffin: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Paxillin stained by Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-GT712-Immunohistochemistry-Paraffin-NBP3-13552-img0002.jpg)
![Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-GT712-Western-Blot-NBP3-13552-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-GT712-Western-Blot-NBP3-13552-img0003.jpg)