| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56508SS | |||
| Azide and BSA Free | NBP2-33213 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24702SS | |||
| Azide and BSA Free | NBP2-33256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85771-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82523-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81829-25ul | |||
![Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Western-Blot-NB100-56508-img0003.jpg "Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml."){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-BSA-Free-Flow-Cytometry-NB100-56508-img0012.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0005.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0009.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-BSA-Free-Flow-Cytometry-NB100-56508-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0009.jpg "Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence."){kind=icon}
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NBP2-24702) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Dual-RNAscope-ISH-IHC-NBP2-24702-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0014.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0001.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0013.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody [NB100-56396] - Analysis of RANK in RAW cell lysate.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-Western-Blot-NB100-56396-img0003.jpg "Western Blot: RANK/TNFRSF11A Antibody [NB100-56396] - Analysis of RANK in RAW cell lysate."){kind=icon}
![Immunohistochemistry-Paraffin: RANK/TNFRSF11A Antibody [NBP1-85771] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-Immunohistochemistry-Paraffin-NBP1-85771-img0002.jpg "Immunohistochemistry-Paraffin: RANK/TNFRSF11A Antibody [NBP1-85771] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons."){kind=icon}
![Western Blot: BCAR3 Antibody [NBP1-82523] - Analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NBP1-82523-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: BCAR3 Antibody [NBP1-82523] - Analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BCAR3 Antibody [NBP1-82523] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and centrosome.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82523-img0014.jpg)
![Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523] - Staining of human kidney shows moderate cytoplasmic positivity in tubular cells.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Immunohistochemistry-Paraffin-NBP1-82523-img0013.jpg)
![Western Blot: OSTM1 Antibody [NBP1-81829] - Analysis in control (vector only transfected HEK293T lysate) and OSTM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/OSTM1-Antibody-Western-Blot-NBP1-81829-img0005.jpg "Western Blot: OSTM1 Antibody [NBP1-81829] - Analysis in control (vector only transfected HEK293T lysate) and OSTM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: OSTM1 Antibody [NBP1-81829] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/OSTM1-Antibody-Immunohistochemistry-Paraffin-NBP1-81829-img0002.jpg)
![Western Blot: BCAR3 Antibody [NBP2-81789] - Analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NBP2-81789-img0001.jpg "Western Blot: BCAR3 Antibody [NBP2-81789] - Analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: BCAR3 Antibody - BSA Free [NBP2-81789] - Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81789_rabbit-polyclonal-bcar3-antibody-2711202419592018.jpg)
![Western Blot: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NB100-87003-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody.")
![Western Blot:[NBP3-28991]-Detection of human RANK by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T , HeLa and Jurkat cells. Antibodies: Affinity purified rabbit anti-RANK antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. RANK was also immunoprecipitated by rabbit anti-RANK antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds and 30 seconds.](http://images.novusbio.com/fullsize/nbp3-28991_rabbit-rank-tnfrsf11a-pab-23120251225633.jpg "Western Blot:[NBP3-28991]-Detection of human RANK by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T , HeLa and Jurkat cells. Antibodies: Affinity purified rabbit anti-RANK antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. RANK was also immunoprecipitated by rabbit anti-RANK antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds and 30 seconds."){kind=icon}
![Western Blot:[NBP3-28991]-RANK/TNFRSF11A Antibody](http://images.novusbio.com/fullsize/nbp3-28991_rabbit-rank-tnfrsf11a-pab-31120250364025.jpg){kind=icon}
![Western Blot: BCAR3 Antibody [NB300-825] - Analysis of BCAR3 in Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NB300-825-img0003.jpg "Western Blot: BCAR3 Antibody [NB300-825] - Analysis of BCAR3 in Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
