| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71860 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54551-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73452 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74847 | |||
![Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Western-Blot-NBP2-01128-img0008.jpg "Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-01128-img0007.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Human lymphoma tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0006.jpg)
![Flow Cytometry: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANAPC2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Flow-Cytometry-NBP2-01128-img0001.jpg)
![Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Western-Blot-NBP2-01128-img0002.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0003.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0004.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0005.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0001.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0004.jpg)
![Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Protein-Array-NBP2-53354-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53354_mouse-monoclonal-e-cadherin-antibody-cdh1-1525-17720241044616.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0015.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0014.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP2-20053-img0010.jpg)
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242063390.jpg)
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-NBP1-21367-img0006.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP1-21367-img0003.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0004.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0005.jpg)
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0007.jpg "Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0004.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS,OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cellsprepared using NETN lysis buffer. Antibody: Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP3-14687] - Whole cell lysate(1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody. For blotting immunoprecipitated E-Cadherin, was used at 1:1000.Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP3-14687-img0001.jpg)
![Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Western-Blot-NBP2-02275-img0003.jpg "Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.")
![Immunocytochemistry/Immunofluorescence: PKMYT1 Antibody (OTI5E1) [NBP2-02275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP2-02275-img0014.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human thyroid tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0012.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Jurkat cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0002.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0004.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0005.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0006.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0007.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human endometrium tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0008.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human Kidney tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0009.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human liver tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0010.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human prostate tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0011.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Hela cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0001.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg)
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg)
![HRAS Antibody (OTI1D9)[NBP2-45745]Simple Western� analysis of endogenous protein MCAM from HELA lysates (0.1 mg/mL) using CD146 (MCAM) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45745_mouse-hras-mab-oti1d9-simple-western-131120251236222.jpg%253fstore=america&image-type=image)