| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98309-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38306-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14052-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89921-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73301 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03712-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15589-20ul | |||
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-98309-img0006.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunochemical staining of mouse 14-3-3 sigma/Stratifin in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-98309-img0004.jpg)
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Mouse 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 ul anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-98309-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98309 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98309_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520252135137.png)
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82097_rabbit-polyclonal-p38-alpha-p-tyr182-antibody-10320252332107.jpg)
![Immunocytochemistry/Immunofluorescence: SLC39A10 Antibody [NBP2-38306] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SLC39A10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38306-img0002.jpg "Immunocytochemistry/Immunofluorescence: SLC39A10 Antibody [NBP2-38306] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: SLC39A10 Antibody [NBP2-38306] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/antibody/nbp2-38306_rabbit-polyclonal-slc39a10-antibody-immunohistochemistry-paraffin-158202484013..jpg)
![Immunohistochemistry-Paraffin: SLC39A10 Antibody [NBP2-38306] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-38306_rabbit-polyclonal-slc39a10-antibody-immunohistochemistry-paraffin-158202484124..jpg)
![Immunohistochemistry-Paraffin: SLC39A10 Antibody [NBP2-38306] - Staining of human fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-38306_rabbit-polyclonal-slc39a10-antibody-immunohistochemistry-paraffin-158202484239..jpg)
![Immunohistochemistry-Paraffin: SLC39A10 Antibody [NBP2-38306] - Staining of human liver shows strong membranous positivity in cholangiocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-38306_rabbit-polyclonal-slc39a10-antibody-immunohistochemistry-paraffin-158202484436..jpg)
![Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human stomach shows moderate to strong positivity in mucus in glandular cells.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0009.jpg "Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human stomach shows moderate to strong positivity in mucus in glandular cells.")
![Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Analysis in human stomach and testis tissues. Corresponding GKN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0006.jpg)
![Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0007.jpg)
![Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0008.jpg)
![Immunohistochemistry-Paraffin: Gastrokine 1 Antibody [NBP2-14052] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14052-img0010.jpg)
![Immunohistochemistry-Paraffin: CXCL7/NAP-2 Antibody [NBP1-89921] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89921-img0006.jpg "Immunohistochemistry-Paraffin: CXCL7/NAP-2 Antibody [NBP1-89921] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.")
![Immunohistochemistry-Paraffin: CXCL7/NAP-2 Antibody [NBP1-89921] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89921-img0005.jpg)
![Immunohistochemistry-Paraffin: CXCL7/NAP-2 Antibody [NBP1-89921] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89921-img0007.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp1-89921_rabbit-polyclonal-cxcl7-nap-2-antibody-15520258303395.jpg)
![Western Blot: Somatostatin R4/SSTR4 Antibody [NB100-74539] - Analysis of 25 ug of HepG2 (lane 1), Jurkat (lane 2) and NIH-3T3 (lane 3) cell lysates.](http://images.novusbio.com/fullsize/Somatostatin-R4-SSTR4-Antibody-Western-Blot-NB100-74539-img0006.jpg "Western Blot: Somatostatin R4/SSTR4 Antibody [NB100-74539] - Analysis of 25 ug of HepG2 (lane 1), Jurkat (lane 2) and NIH-3T3 (lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Somatostatin R4/SSTR4 Antibody [NB100-74539] - Analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Somatostatin-R4-SSTR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74539-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Somatostatin R4/SSTR4 Antibody [NB100-74539] - Analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Somatostatin-R4-SSTR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74539-img0004.jpg)
![Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP1-77894-img0002.jpg "Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.")
![Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-NBP1-77894-img0004.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Western Blot: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.](http://images.novusbio.com/fullsize/PDF-Antibody-3F3-Western-Blot-NBP2-00814-img0004.jpg "Western Blot: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.")
![Immunocytochemistry/Immunofluorescence: PDF Antibody (3F3) [NBP2-00814] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.](http://images.novusbio.com/fullsize/PDF-Antibody-3F3-Immunocytochemistry-Immunofluorescence-NBP2-00814-img0005.jpg)
![Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PDF-Antibody-3F3-Flow-Cytometry-NBP2-00814-img0003.jpg)
![Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Hela cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PDF-Antibody-3F3-Flow-Cytometry-NBP2-00814-img0001.jpg)
![Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Jurkat cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PDF-Antibody-3F3-Flow-Cytometry-NBP2-00814-img0002.jpg)
![Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP3-03712-img0004.jpg "Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded mouse heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0003.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded rat heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0001.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded human colon carcinoma using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0002.jpg)
![Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-3-Antibody-Western-Blot-NBP3-15589-img0001.jpg "Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunofluorescence analysis of Hep G2 cells using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517361286.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517311036.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517303110.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517352770.jpg)