| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27073SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47768-0.1mg | |||
| IHC-Prediluted | NBP2-48153 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54476-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03978SS | |||
![Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-289-img0010.jpg)
![Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0002.jpg)
![Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-289_rabbit-polyclonal-mcm3-antibody-western-blot-2122024155814..jpg)
![Western Blot: Calpain 1 Antibody [NBP2-15671] - Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Western-Blot-NBP2-15671-img0006.jpg "Western Blot: Calpain 1 Antibody [NBP2-15671] - Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15671] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15671-img0004.jpg)
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671] - Mouse lung. Calpain 1 antibody [N3C2], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0007.jpg)
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0003.jpg)
![Immunoprecipitation: Calpain 1 Antibody [NBP2-15671] - Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 ug of Calpain 1 antibody. Western blot analysis was performed using Calpain 1 antibody.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunoprecipitation-NBP2-15671-img0005.jpg)
![Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0011.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SNF5 Antibody [NB100-57525] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-57525 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCB1/SNF5 was also immunoprecipitated by another rabbit anti-SMARCB1/SNF5 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0002.jpg)
![Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0010.jpg)
![Immunoprecipitation: SNF5 Antibody [NB100-57525] - Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 (lot NB100-57525-2) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody NB100-57525-1. For blotting immunoprecipitated SMARCB1/SNF5, NB100-57525 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunoprecipitation-NB100-57525-img0014.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-27073-img0004.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-27073-img0003.jpg)
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (57P06) [NBP2-44492] - Human Prostate Carcinoma stained with p57 Kip2 Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-57P06-Immunohistochemistry-Paraffin-NBP2-44492-img0002.jpg "Immunohistochemistry-Paraffin: p57 Kip2 Antibody (57P06) [NBP2-44492] - Human Prostate Carcinoma stained with p57 Kip2 Antibody (KIP2/880).")
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).")
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0001.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg "Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-26112025182908.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251843456.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251850424.jpg)
![Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser318]-Antibody-Western-Blot-NBP2-03978-img0003.jpg "Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.")
![Western Blot: Cdk5 Antibody [NBP2-15843] - Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Cdk5-Antibody-Western-Blot-NBP2-15843-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody [NBP2-15843] - Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cdk5 Antibody [NBP2-15843] - Analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15843-img0004.jpg)
![Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-15843] - Rat hind brain. CDK5 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunohistochemistry-Paraffin-NBP2-15843-img0005.jpg)
![Western Blot: Cdk5 Antibody [NBP2-15843] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Western-Blot-NBP2-15843-img0006.jpg)
![Western Blot: Cdk5 Antibody [NBP2-15843] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Cdk5-Antibody-Western-Blot-NBP2-15843-img0008.jpg)
![Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-15843] - ES2 xenograft, using CDK5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunohistochemistry-Paraffin-NBP2-15843-img0003.jpg)
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunocytochemistry-Immunofluorescence-H00010445-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on formalin-fixed paraffin-embedded human spleen. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunohistochemistry-Paraffin-H00010445-B01P-img0005.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Analysis of MCRS1 expression in transfected 293T cell line by MCRS1 polyclonal antibody. Lane 1: MCRS1 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-H00010445-B01P-img0006.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Identification of interacting partners of PRDM9 by Y2H screening. a Schematic representation of the domain structure of seven PRDM9-interacting proteins identified by Y2H screening. The prey fragments identified in the screens are shown as blue bars underneath each protein. The length (number of amino acid residues) of each full-length protein is indicated on the right. b RNA expression of the PRDM9-interacting proteins in mouse testes (left) & ovaries (right) during meiosis. Data were extracted from RNA-seq data in Margolin et al. (2014) & Soh et al. (2015)). The average gene expression is shown in reads per kilobase per million of reads (RPKM) & fragments per kilobase of transcript per million mapped read (FKPM) for testes & ovaries, respectively. Zoomed-in views of the dashed line boxes are shown below. Spo11 & Spata19 expression were used as positive & negative controls, respectively. c Western blot analysis of PRDM9, CXXC1, PIH1D1, CEP70, & MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, & 15.5 dpp & adult mice. Each lane was loaded with 50 μg of testis protein extracts. Tubulin alpha was used as internal control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28527011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010445-b01p_mouse-polyclonal-mcrs1-antibody-310202415483646.jpg)