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![Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0012.jpg "Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74608] - Human colon carcinoma peyer's patch. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74608-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74608] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74608. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74609 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0011.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - SUMO2-PCNA enriches CAF1 & FACT within the replication complex. a Western blot analysis of the indicated proteins in the CB fractions (left) & the FLAG-PCNA complexes purified from CB fractions (right) prepared from cells expressing the indicated FLAG-tagged proteins. Ub-KR indicates the FLAG-ubiquitin-PCNA (KR) fusion protein. b Western blot analysis of the indicated proteins in the CB input (left panels) used for the in vitro pull-down experiments of purified recombinant strep-tagged PCNA, SUMO2-conjugated PCNA, & GFP anchored to Strep-Tactin resins (right panels). SUMO2-modified PCNA was generated by the in vitro SUMOylation reaction using strep-PCNA & His-SUMO2 shown in Supplementary Fig. 6a, b. c Western blot analysis of the input levels of recombinant strep-PCNA & strep-SUMO2-PCNA fusion (S2-PCNA) purified from E. coli (left panel) & of strep-PCNA & strep-S2-PCNA pulled down by WT or SIM mutant FLAG-CAF1A proteins purified & anchored on FLAG M2 agarose beads from HEK293T cells expressing the corresponding constructs (right panels). d Western blot analysis of strep-S2-PCNA (upper 2 panels) & strep-PCNA (lower 2 panels) pulled down by FLAG-CAF1A purified from E. coli & anchored on FLAG M2 agarose beads. e Western blot analysis of the indicated proteins in the CB input prepared from HEK293T cells expressing FLAG-SSRP1 WT or SIM mutant (left panels) & of FLAG-SSRP1 & SPT16 pulled down from the CB fractions in vitro using purified recombinant strep-tagged PCNA, S2-PCNA, & GFP anchored to Strep-Tactin resins (right panels) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30006506), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74608_rabbit-polyclonal-chaf1a-antibody-310202416575.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Western-Blot-NBP2-33320-img0017.jpg "Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Immunohistochemistry-Paraffin-NBP2-33320-img0015.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Flow-Cytometry-NBP2-33320-img0018.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0007.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0009.jpg)
![Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Protein-Array-NBP2-33320-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunocytochemistry-Immunofluorescence-NBP2-44735-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0007.jpg)
![Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - An intracellular stain was performed on K562 cells with Ornithine Decarboxylase [ODC1/487] Antibody NBP2-47837AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Flow-Cytometry-NBP2-44735-img0004.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0001.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0006.jpg)
![Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunohistochemistry-Paraffin-NBP1-33506-img0003.jpg "Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-NBP1-33506-img0007.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-2611202518191318.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251830124.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251829022.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-26112025196217.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-23891-img0002.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.")
![Flow Cytometry: Dicer Antibody [NBP2-23891] - Analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.](http://images.novusbio.com/fullsize/Dicer-Antibody-Flow-Cytometry-NBP2-23891-img0003.jpg)
![ELISA: Dicer Antibody [NBP2-23891] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-ELISA-NBP2-23891-img0001.jpg)
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-NBP1-25966-img0017.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.")
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0020.jpg)
![Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Western-Blot-H00051053-M01-img0012.jpg "Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunocytochemistry-Immunofluorescence-H00051053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunohistochemistry-Paraffin-H00051053-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunohistochemistry-Paraffin-H00051053-M01-img0008.jpg)
![ELISA: Geminin Antibody (1A8) [H00051053-M01] - Detection limit for recombinant GST tagged GMNN is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-ELISA-H00051053-M01-img0013.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0005.jpg "Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.")
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Analysis of ZFYVE21 expression in transfected 293T cell line by ZFYVE21 polyclonal antibody. Lane 1: ZFYVE21 transfected lysate(25.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 and complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30min, 25% v/v in gelatin veronal buffer) or LIGHT (12h, 50ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n=12) were stained as indicated. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0003.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Recombinant FLAG-tagged ZFYVE21 (1ug) and/or recombinant SMURF2 (1ug) protein was incubated for 30min at room temperature and analyzed by western blot. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0004.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 & complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30 min, 25% v/v in gelatin veronal buffer) or LIGHT (12 h, 50 ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n = 12) were stained as indicated (b). Synovial biopsies from osteoarthritis (n = 11), low-titer rheumatoid factor antibody rheumatoid arthritis (RF RA; n = 8), & high-titer RF RA (n = 8) were stained as indicated (c–e) & spatial correlations between ZFVYE21 or nuclear factor-inducing kinase (NIK) with endothelial cells (Ulex, f) & membrane attack complex (polyC9, g) were assessed using the computer software (scale bar: 200 μm). ****p < 1 × 10−5, Student’s t test. CD45 abundance was assessed (h) & correlated with NIK staining (i) via blinded scoring from three independent observers. *p < 0.05, **p < 0.001. Western blotting was repeated three times. Analysis of variance statistical analyses were performed. Representative data shown *p < 0.05, **p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-31020241548367.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - SMURF2-dependent degradation of vesicular PTEN. PTEN co-immunoprecipitations (co-IPs) were performed on human umbilical vein endothelial cells (HUVECs) treated for 30 min with panel reactive antibody (PRA) sera & blotted as indicated (a). Rab5 co-IPs were performed on PRA-treated HUVECs transfected with control or SMURF2 siRNA (b). Western blot analysis of whole-cell lysates of HUVECs transiently transfected with SMURF2 WT, SMURF2 DN, control siRNA, & SMURF2 siRNA (c). HUVECs transfected with control, SMURF2, or ZFYVE21 siRNA were treated with PRA & Rab5 co-IPs were performed (d). Recombinant FLAG-tagged ZFYVE21 (1 μg) and/or recombinant SMURF2 (1 μg) protein was incubated for 30 min at room temperature & analyzed by western blot (e). In vitro kinase assays were performed using various E2 ubiquitin ligases with SMURF2 protein (1 μg) as the E3 ubiquitin ligase & PTEN (1 μg) as substrate (f). In vitro kinase assays were performed using isolated SMURF2 WT or SMURF2 DN as E3 ubiquitin ligases, UbcH5c as the E2 ubiquitin ligase, & PTEN as substrate as indicated (g). HUVECs were pretreated with MG132 for 4 h or chloroquine for 30 min prior to PRA treatment at the indicated times prior to Rab5 co-IP & western blot analysis (h). Western blot analyses were repeated two to four times. Confocal microscopic studies analyzed ≥4 cells per group & was repeated three times. In vitro kinase assays were repeated twice. Representative data shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-310202415485117.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in NIH/3T3.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0014.jpg "Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of monoclonal antibody to NEK9 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Immunocytochemistry-Immunofluorescence-H00091754-M01-img0009.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6 Analysis of NEK9 expression in HeLa.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0011.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in PC-12.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0012.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6. Analysis of NEK9 expression in Raw 264.7.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0013.jpg)
![Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - Analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody (M01), clone 1F6.Lane 1: NEK9 transfected lysate(107.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Western-Blot-H00091754-M01-img0015.jpg)
![Sandwich ELISA: NEK9 Antibody (1F6) [H00091754-M01] - Detection limit for recombinant GST tagged NEK9 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK9-Antibody-1F6-Sandwich-ELISA-H00091754-M01-img0010.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0016.jpg "Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Immunohistochemistry-Paraffin-H00001445-M01-img0010.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0012.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3 Analysis of CSK expression in HL-60.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0014.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3. Analysis of CSK expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0015.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0017.jpg)
![ELISA: CSK Antibody (3A3) [H00001445-M01] - Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-ELISA-H00001445-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunocytochemistry-Immunofluorescence-H00003833-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunohistochemistry-Paraffin-H00003833-M01-img0007.jpg)
![ELISA: KIFC1 Antibody (2B9) [H00003833-M01] - Detection limit for recombinant GST tagged KIFC1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-ELISA-H00003833-M01-img0009.jpg)