DDX24 Antibody

Western Blot: DDX24 Antibody [NB100-2226] - Detection of Human DDX24 on HeLa whole cell lystate using NB100-2226. DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody NB100-2225 and NB100-2225.

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX24 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: DDX24 Antibody [NB100-2226] - Staining of paraffin embedded human liver tissue with at a dilution of 1:100, detected using HRP/DAB detection system, counterstained with hematoxylin. Epitope retrieval, HIER with Tris/EDTA (pH 9.0) buffer using pressure cooker for 20 mins. Diluted using TBS with 5% BSA and incubated at 4 degrees C overnight. Image submitted by a verified customer review.

• Reactivity: Hu, Pm-Or
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX24 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 800 and the C-terminus (residue 859) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062).
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. DDX24 antibody validated for IHC-P from a verified customer review.
• Control:

  DDX24 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX24 Antibody

• ATP-dependent RNA helicase DDX24
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• DEAD box protein 24
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24
• EC 3.6.1
• EC 3.6.4.13
• S. cerevisiae CHL1-like helicase

Background

DDX24 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DDX24 has not been elucidated. Alternate names for DDX24 include ATP-dependent RNA helicase DDX24, and DEAD box protein 24.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DDX24 Antibody (NB100-2226) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Haopei Zhang IHC-P Human 02/21/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver
• Species: Human
• Lot: A1

Comments

• Comments: Epitope retrieval: HIER with Tris/EDTA (pH=9.0) buffer using pressure cooker for 20 mins.
  Primary antibody: Diluted using TBS with 5% BSA at 1/100, incubate at 4°C overnight.
  Detection system: HRP/DAB

Bioinformatics

• Gene Symbol: DDX24
• Entrez:
  • 57062(Human)
• Uniprot:
  • NP_065147.1(Human)