| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56394SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
![Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Western-Blot-NBP2-76412-img0001.jpg "Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE HCT 116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunocytochemistry-NBP2-76412-img0004.jpg)
![Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunohistochemistry-NBP2-76412-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (NBP2-76412) and rabbit anti-SMC1 (NB100-204).](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunoprecipitation-NBP2-76412-img0005.jpg)
![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-2120249393515.jpg "Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939177.jpg)
![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939148.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024941456.jpg)
![Immunoprecipitation of Rad21 protein from HeLa whole cell extract using 5 ug of Rad21 antibody [HL2171] (NBP3-25619).Western blot analysis was performed using Rad21 antibody [HL2171] (NBP3-25619).EasyBlot HRP-conjugated anti rabbit IgG antibody .](http://images.novusbio.com/fullsize/nbp1-32470_rabbit-polyclonal-p2x7-p2rx7-antibody-immunohistochemistry-frozen-141120251855419.jpg)
![Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Western-Blot-NBP2-46199-img0006.jpg "Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.")
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0005.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0001.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0002.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0003.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0004.jpg)
![Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Western-Blot-NBP3-15798-img0004.jpg "Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunoprecipitation-NBP3-15798-img0005.jpg)
![Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Western-Blot-NBP1-51596-img0007.jpg "Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunocytochemistry-Immunofluorescence-NBP1-51596-img0010.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunohistochemistry-Paraffin-NBP1-51596-img0008.jpg)
![Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Flow-Cytometry-NBP1-51596-img0009.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")
![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)
![Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MIS12-Antibody-Western-Blot-NB100-2359-img0006.jpg "Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). Detection: DAB](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunohistochemistry-NB100-2359-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody NB100-2358. For blotting immunoprecipitated MIS12, NB100-2359 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunoprecipitation-NB100-2359-img0005.jpg)
![Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0002.jpg)
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")