| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50055SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55870-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59319-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26415-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21185-25ul | |||
| Azide and BSA Free | NBP3-44312 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21184-25ul | |||
| Azide and BSA Free | NBP3-44311 | |||
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Immunocytochemistry-Immunofluorescence-NBP3-13617-img0002.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0003.jpg)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13617_mouse-hsp27-p-ser-82-mab-gt357-western-blot-2611202518484816.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055] - Analysis of a region of cerebral cortex from an Alzheimer's disease (AD) patient stained with NBP2-50055, the signal detected with HRP and DAB. Senile plaques are labeled SP. The region of the lowest of the three plaques is shown in the inset stained with the fluorescent dye thioflavin-S. This dye binds to not only the senile plaque but also a neurofibrillary tangle (NFT), the other pathological hallmark of AD, which does not contain ABeta.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-AB9-NBP2-50055-img0005.jpg "Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055] - Analysis of a region of cerebral cortex from an Alzheimer's disease (AD) patient stained with NBP2-50055, the signal detected with HRP and DAB. Senile plaques are labeled SP. The region of the lowest of the three plaques is shown in the inset stained with the fluorescent dye thioflavin-S. This dye binds to not only the senile plaque but also a neurofibrillary tangle (NFT), the other pathological hallmark of AD, which does not contain ABeta.")
![Western Blot: beta Amyloid Antibody (AB9) [NBP2-50055] - Amyloid beta peptide blotted with NBP2-50055. NBP2-50055 recognizes amyloid beta peptide running at a molecular weight of 5 kDa and amyloid beta aggregates.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-AB9-Western-Blot-NBP2-50055-img0004.jpg)
![Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-3B9-Western-Blot-H00005473-M01-img0006.jpg "Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-3B9-Western-Blot-H00005473-M01-img0004.jpg)
![Sandwich ELISA: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CXCL7-NAP-2-Antibody-3B9-Sandwich-ELISA-H00005473-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-55870] - Staining in human cerebral cortex and pancreas tissues using anti-CDK5 antibody. Corresponding CDK5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunohistochemistry-Paraffin-NBP2-55870-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-55870] - Staining in human cerebral cortex and pancreas tissues using anti-CDK5 antibody. Corresponding CDK5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cdk5 Antibody [NBP2-55870] - Staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55870-img0001.jpg)
![Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-55870] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunohistochemistry-Paraffin-NBP2-55870-img0002.jpg)
![Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-55870] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cdk5-Antibody-Immunohistochemistry-Paraffin-NBP2-55870-img0003.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Immunoprecipitation: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Immunoprecipitating Jak2 [p Tyr1007, p Tyr1008] in Hela whole cell lysate treated with Pervanadate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26415 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26415 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg). Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26415_rabbit-jak2-p-tyr-1007-p-tyr-1008-mab-1a4-262202413221522.jpg "Immunoprecipitation: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Immunoprecipitating Jak2 [p Tyr1007, p Tyr1008] in Hela whole cell lysate treated with Pervanadate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26415 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26415 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg). Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
![Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Image of Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26415_rabbit-jak2-p-tyr-1007-p-tyr-1008-mab-1a4-262202414471549.jpg)
![Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Image of Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26415_rabbit-jak2-p-tyr-1007-p-tyr-1008-mab-1a4-27220248274910.jpg)
![Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not). All lanes: Jak2 [p Tyr1007, p Tyr1008] Antibody at 0.75ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa Observed band size: 120 KDa](http://images.novusbio.com/fullsize/nbp3-26415_rabbit-jak2-p-tyr-1007-p-tyr-1008-mab-1a4-282202411413613.jpg)
![Western Blot: Jak2 Antibody [NBP1-22975] - Whole cell lysate from Jurkat (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAK2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. JAK2 was also immunoprecipitated by rabbit anti-JAK2 antibody BL3496, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Jak2-Antibody-Western-Blot-NBP1-22975-img0004.jpg "Western Blot: Jak2 Antibody [NBP1-22975] - Whole cell lysate from Jurkat (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAK2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. JAK2 was also immunoprecipitated by rabbit anti-JAK2 antibody BL3496, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Immunohistochemistry-Paraffin: Jak2 Antibody [NBP1-22975] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAK2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Jak2-Antibody-Immunohistochemistry-Paraffin-NBP1-22975-img0003.jpg)
