| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89839-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71239 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15608-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB6000-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89840-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85217-25ul | |||
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Western-Blot-NB500-191-img0010.jpg "Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Immunoprecipitation-NB500-191-img0011.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0015.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0014.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP2-20053-img0010.jpg)
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242063390.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human cerebral cortex, fallopian tube, placenta and testis using Anti-RSPH1 antibody NBP1-89839 (A) shows similar protein distribution across tissues to independent antibody NBP1-89840 (B).](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89839-img0015.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human cerebral cortex, fallopian tube, placenta and testis using Anti-RSPH1 antibody NBP1-89839 (A) shows similar protein distribution across tissues to independent antibody NBP1-89840 (B).")
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89839-img0017.jpg)
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89839-img0020.jpg)
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89839-img0019.jpg)
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89839] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89839-img0018.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0007.jpg "Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0006.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0002.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0005.jpg)
![Immunofluorescence: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunofluorescence-NBP2-45814-img0001.jpg)
![Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of C6 cells, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-15608-img0006.jpg "Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of C6 cells, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0003.jpg)
![Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of various cell lines, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-15608-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0002.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP3-15608] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 antibody (NBP3-15608). Western blot was performed from the immunoprecipitate using Rad21 antibody (NBP3-15608) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP3-15608-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Rad21 Antibody - Azide and BSA Free [Rad21] - Immunofluorescence analysis of U2OS cells using Rad21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15608_rabbit-rad21-pab-52202517311014.jpg)
![Chromatin Immunoprecipitation: Rad21 Antibody - Azide and BSA Free [Rad21] - Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15608_rabbit-rad21-pab-52202517311077.jpg)
![Immunoprecipitation: Rad21 Antibody - Azide and BSA Free [Rad21] - Immunoprecipitation analysis of 300 ug extracts of Jurkat cells using 3 ug Rad21 antibody . Western blot was performed from the immunoprecipitate using Rad21 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15608_rabbit-rad21-pab-5220251730318.jpg)
![Chromatin Immunoprecipitation: Rad21 Antibody - Azide and BSA Free [Rad21] - Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15608_rabbit-rad21-pab-52202517363718.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0002.jpg "Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71239-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0007.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0001.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0005.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0006.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Western-Blot-MAB6000-img0002.jpg "Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.")
![Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Immunohistochemistry-MAB6000-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - An intracellular stain was performed on HeLa cells with Cyclin B1 Antibody [2061A] MAB6000F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0004.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - K562 human cell line was stained with Rabbit Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB6000, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0001.jpg)
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89840] - Analysis in human fallopian tube and cerebral cortex tissues using NBP1-89840 antibody. Corresponding RSPH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89840-img0022.jpg)
![Immunohistochemistry-Paraffin: TSGA2 Antibody [NBP1-89840] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/TSGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89840-img0023.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DIAPH-2 Antibody [NBP1-85217] - Staining of human cell line U-251 MG shows localization to nucleoli, endoplasmic reticulum and vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85217-img0016.jpg)
![Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0013.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0014.jpg)