| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70775 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87245-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73481 | |||
![Western Blot: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep22788) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0018.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-31336-img0009.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A. 50 ug mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0010.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A.50 ug rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-31336] - Fumarase antibody detects Fumarase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarase stained by Fumarase antibody (NBP1-31336) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31336_rabbit-polyclonal-fumarase-antibody-910202420122069.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: Frk Antibody (OTI6D2) [NBP1-47761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Western-Blot-NBP1-47761-img0001.jpg "Western Blot: Frk Antibody (OTI6D2) [NBP1-47761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.")
![Immunocytochemistry/Immunofluorescence: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunocytochemistry-Immunofluorescence-NBP1-47761-img0009.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0008.jpg)
![Flow Cytometry: Frk Antibody (OTI6D2) [NBP1-47761] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FRK antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Flow-Cytometry-NBP1-47761-img0010.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0002.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0003.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0004.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0005.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0006.jpg)
![Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Immunohistochemistry-Paraffin-NBP1-47761-img0007.jpg)
![Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Western-Blot-NBP2-70775-img0001.jpg "Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.")
![Immunocytochemistry/Immunofluorescence: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70775-img0003.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0009.jpg)
![Flow Cytometry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FRK antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70775-img0002.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0004.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0005.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0006.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0007.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0008.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0010.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87245-img0014.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0018.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0009.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0008.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0011.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-PPM1G antibody NBP1-87245 (A) shows similar protein distribution across tissues to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0015.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human kidney.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0017.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89814-img0015.jpg "Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse hippocampus shows neuronal immunoreactivity in the CA3 layer.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342022.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse globus pallidus shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133407.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse medulla shows positivity in the neurons of the facial nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342036.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse olfactory bulb shows neuronal positivity in the anterior olfactory nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334012.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342060.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334201.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133402.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342070.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0018.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NB110-38867-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0005.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:500.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0001.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:100.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0002.jpg)
![Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0004.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20054-img0002.jpg "Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: RAD50 Antibody [NBP2-20054] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20054-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rad50 Antibody [NBP2-20054] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20054-img0003.jpg)
![Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20054_rabbit-polyclonal-rad50-antibody-western-blot-261120251819362.jpg)
![Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-20054_rabbit-polyclonal-rad50-antibody-western-blot-261120251821106.jpg)
![Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/CtIP-Antibody-Western-Blot-NB100-79810-img0006.jpg "Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CtIP Antibody [NB100-79810] - Detection of human CtIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CtIP antibody NB100-79810 used for IP at 3 ug per reaction. CtIP was also immunoprecipitated by rabbit anti-CtIP antibody NB100-79809. For blotting immunoprecipitated CtIP, NB100-79810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CtIP-Antibody-Immunoprecipitation-NB100-79810-img0007.jpg)
![Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Western-Blot-NBP2-46459-img0008.jpg "Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.")
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0007.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0002.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0003.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0004.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0005.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0006.jpg)
![Immunofluorescence: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunofluorescence-NBP2-46459-img0001.jpg)