| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92902-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33282-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37935-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55409-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88156-25ul | |||
![Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP2-92902-img0005.jpg "Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded rat brain using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human colon carcinoma using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human lung cancer using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0003.jpg)
![Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP1-18878-img0004.jpg "Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-18878-img0003.jpg)
![Immunoprecipitation: Cyclin C Antibody [NBP1-18878] - Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody NBP1-18878 used for IP at 6 ug per reaction. For blotting immunoprecipitated Cyclin C, NBP1-18878 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunoprecipitation-NBP1-18878-img0005.jpg)
![Western Blot: ERH Antibody (4A10) [H00002079-M14] - Analysis of ERH expression in transfected 293T cell line by ERH monoclonal antibody (M14), clone 4A10. Lane 1: ERH transfected lysatE (12.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Western-Blot-H00002079-M14-img0006.jpg "Western Blot: ERH Antibody (4A10) [H00002079-M14] - Analysis of ERH expression in transfected 293T cell line by ERH monoclonal antibody (M14), clone 4A10. Lane 1: ERH transfected lysatE (12.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ERH Antibody (4A10) [H00002079-M14] - Analysis of monoclonal antibody to ERH on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Immunocytochemistry-Immunofluorescence-H00002079-M14-img0003.jpg)
![Immunohistochemistry-Paraffin: ERH Antibody (4A10) [H00002079-M14] - Analysis of monoclonal antibody to ERH on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Immunohistochemistry-Paraffin-H00002079-M14-img0005.jpg)
![ELISA: ERH Antibody (4A10) [H00002079-M14] - Detection limit for recombinant GST tagged ERH is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-ELISA-H00002079-M14-img0002.jpg)
![Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining in human placenta and liver tissues using NBP2-33282 antibody. Corresponding EGFL6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EGF-L6-Antibody-Immunohistochemistry-Paraffin-NBP2-33282-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining in human placenta and liver tissues using NBP2-33282 antibody. Corresponding EGFL6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/EGF-L6-Antibody-Immunohistochemistry-Paraffin-NBP2-33282-img0005.jpg)
![Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/EGF-L6-Antibody-Immunohistochemistry-Paraffin-NBP2-33282-img0006.jpg)
![Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining of human placenta shows moderate membranous/ cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGF-L6-Antibody-Immunohistochemistry-Paraffin-NBP2-33282-img0007.jpg)
![Immunohistochemistry-Paraffin: EGF-L6 Antibody [NBP2-33282] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/EGF-L6-Antibody-Immunohistochemistry-Paraffin-NBP2-33282-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NFIC Antibody [NBP2-37935] - Staining of human cell line MCF7 shows positivity in nucleus and nucleoli.](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37935-img0001.jpg "Immunocytochemistry/Immunofluorescence: NFIC Antibody [NBP2-37935] - Staining of human cell line MCF7 shows positivity in nucleus and nucleoli.")
![Immunohistochemistry-Paraffin: NFIC Antibody [NBP2-37935] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP2-37935-img0002.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NFIC-Antibody-Western-Blot-NBP1-71879-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71879-img0003.jpg)
![Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71879-img0002.jpg)
![Western Blot: QSOX1/Quiescin Q6 Antibody [NBP2-55409] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/QSOX1-Quiescin-Q6-Antibody-Western-Blot-NBP2-55409-img0002.jpg "Western Blot: QSOX1/Quiescin Q6 Antibody [NBP2-55409] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: QSOX1/Quiescin Q6 Antibody [NBP2-55409] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/QSOX1-Quiescin-Q6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55409-img0001.jpg)
![Western Blot: QSOX1/Quiescin Q6 Antibody [NBP2-55409] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/QSOX1-Quiescin-Q6-Antibody-Western-Blot-NBP2-55409-img0003.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-88156] - Analysis in human cell lines A-431 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-88156-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-88156] - Analysis in human cell lines A-431 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody [NBP1-88156] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88156-img0005.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-88156] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-88156-img0004.jpg)
![Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NB600-495-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NB600-495-img0002.jpg)
![Western Blot: QSOX1/Quiescin Q6 Antibody [NBP3-12338] - CaC12 cells. 1:500 antibody dilution in DiluObuffer. Apparent MW is 90 KDa.](http://images.novusbio.com/fullsize/QSOX1-Quiescin-Q6-Antibody-Western-Blot-NBP3-12338-img0003.jpg "Western Blot: QSOX1/Quiescin Q6 Antibody [NBP3-12338] - CaC12 cells. 1:500 antibody dilution in DiluObuffer. Apparent MW is 90 KDa.")
![Immunohistochemistry-Paraffin: QSOX1/Quiescin Q6 Antibody [NBP3-12338] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/QSOX1-Quiescin-Q6-Antibody-Immunohistochemistry-Paraffin-NBP3-12338-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0005.jpg "Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB")
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0004.jpg)