| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82642-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89503-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84976-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70399 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54551-100ug | |||
![Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NBP1-82642-img0011.jpg "Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: MCM6 Antibody [NBP1-82642] - Staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82642-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining in human lymph node and liver tissues using anti-MCM6 antibody. Corresponding MCM6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-Paraffin-NBP1-82642-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCM6 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NBP1-82642-img0010.jpg)
![Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-Paraffin-NBP1-82642-img0007.jpg)
![Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-Paraffin-NBP1-82642-img0008.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0008.jpg "Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0007.jpg)
![Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunohistochemistry-Paraffin-NBP2-00776-img0006.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0003.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Immunofluorescent staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0005.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0001.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0002.jpg)
![CDK2 Antibody (OTI2D9)[NBP2-00776]Simple Western� analysis of endogenous protein TUBB4A from HELA lysates (0.2 mg/mL) using TUBB4A Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-00776_mouse-cdk2-mab-oti2d9-simple-western-131120251236227.jpg%253fstore=america&image-type=image)
![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0002.jpg "Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0007.jpg)
![Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70392-img0008.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0005.jpg)
![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0006.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0003.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0004.jpg)
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0006.jpg "Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0009.jpg)
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0008.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0011.jpg "Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0004.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0012.jpg)
![Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Flow-Cytometry-NBP2-67899-img0001.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0003.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0005.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0006.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0008.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0009.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16054-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.")
![Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054] - Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 ug of Cyclin D1 antibody [N1C3]. Western blot analysis was performed using Cyclin D1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunoprecipitation-NBP2-16054-img0006.jpg)
![ELISA: Cyclin D1 Antibody [NBP2-16054] - Detection of recombinant full-length Cyclin D1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-ELISA-NBP2-16054-img0014.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420122096.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] - Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Cyclin D1 stained by Cyclin D1 antibody (NBP2-16054) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420104464.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242063359.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242075916.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-1610202419414560.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-western-blot-26112025182002.jpg)
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0010.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunoprecipitation-NBP2-43697-img0002.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunocytochemistry-immunofluorescence-26112025183418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-26112025194811.jpg)
![Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251846105.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.p21 Cip1 stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunohistochemistry-paraffin-2611202518315421.jpg)
![p21 Cip1 antibody [GT1032] (NBP2-43697) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (NBP2-43697) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-flow-cytometry-2611202518321914.jpg)
![Untreated (-) and treated (+) HCT 116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251836336.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89503-img0011.jpg "Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-89503-img0018.jpg)
![Western Blot: ERH Antibody [NBP1-84976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ERH-Antibody-Western-Blot-NBP1-84976-img0007.jpg "Western Blot: ERH Antibody [NBP1-84976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ERH Antibody [NBP1-84976] - Staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/ERH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84976-img0005.jpg)
![Western Blot: ERH Antibody [NBP1-84976] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/ERH-Antibody-Western-Blot-NBP1-84976-img0006.jpg)
![Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Western-Blot-NBP2-01245-img0003.jpg "Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.")
![Immunocytochemistry/Immunofluorescence: CDKN3 Antibody (2E11) [NBP2-01245] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunocytochemistry-Immunofluorescence-NBP2-01245-img0009.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human prostate tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0008.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0002.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0004.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0005.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0006.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human Kidney tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0007.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0001.jpg)
![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0001.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0004.jpg)
![Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Protein-Array-NBP2-53354-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53354_mouse-monoclonal-e-cadherin-antibody-cdh1-1525-17720241044616.jpg)