| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80589 | |||
| BSA Free | NB110-60521SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13657-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93990-0.02ml | |||
![Western Blot: BAP1 Antibody (1G8) [NB110-60521] - Detection of BAP1 in HeLa WCE.](http://images.novusbio.com/fullsize/BAP1-Antibody-1G8-Western-Blot-NB110-60521-img0005.jpg "Western Blot: BAP1 Antibody (1G8) [NB110-60521] - Detection of BAP1 in HeLa WCE.")
![Flow Cytometry: BAP1 Antibody (1G8) [NB110-60521] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using BAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BAP1-Antibody-1G8-Flow-Cytometry-NB110-60521-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-13657] - Staining of human cell line U-251 MG shows localization to nucleoli and centrosome.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13657-img0009.jpg "Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-13657] - Staining of human cell line U-251 MG shows localization to nucleoli and centrosome.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0013.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0010.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0011.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-13657] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-13657-img0012.jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-2120249374941.jpg "Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:250. Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-2120249422615.jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-212024934278.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518405413.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518394124.jpg)
![NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25576_rabbit-nfix-mab-hl1269-azide-and-bsa-free-immunohistochemistry-paraffin-141120251841529.jpg)
![Western Blot: GEF-H1 Antibody (1132) [NBP2-43584] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (1132) diluted at 1:1000. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Western-Blot-NBP2-43584-img0006.jpg "Western Blot: GEF-H1 Antibody (1132) [NBP2-43584] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (1132) diluted at 1:1000. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GEF-H1 Antibody (1132) [NBP2-43584] - GEF-H1 Antibody (1132) detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GEF-H1 stained by GEF-H1 Antibody (1132) diluted at 1:200.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Immunohistochemistry-Paraffin-NBP2-43584-img0005.jpg)
![Western Blot: GEF-H1 Antibody (1132) [NBP2-43584] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [1132] dilution: 1:1000](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Western-Blot-NBP2-43584-img0001.jpg)
![Western Blot: GEF-H1 Antibody (1132) [NBP2-43584] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [1132] dilution: 1:1000](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Western-Blot-NBP2-43584-img0002.jpg)
![Western Blot: GEF-H1 Antibody (1132) [NBP2-43584] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [1132] dilution: 1:1000.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Western-Blot-NBP2-43584-img0003.jpg)
![Immunohistochemistry-Paraffin: GEF-H1 Antibody (1132) [NBP2-43584] - GEF-H1 Antibody (1132) detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GEF-H1 stained by GEF-H1 Antibody (1132) diluted at 1:200.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-1132-Immunohistochemistry-Paraffin-NBP2-43584-img0004.jpg)
![Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-278202415242619.jpg "Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32504) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-298202414262691.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunocytochemistry analysis of Jurkat cells labeling KIFC1 with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202410334073.jpg)
![Flow Cytometry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Flow cytometric analysis of Jurkat cells labeling KIFC1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32504, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202416453263.jpg)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249342764.jpg "Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249432856.jpg)
![Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249371538.jpg)
![Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-212024940870.jpg)
![Western Blot: NFIC Antibody [NBP1-71878] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71879, which recognizes a downstream epitope](http://images.novusbio.com/fullsize/NFIC-Antibody-Western-Blot-NBP1-71878-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFIC Antibody [NBP1-71878] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71879, which recognizes a downstream epitope")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC left image used at a dilution of 1:200 (1ug/ml), right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71878-img0003.jpg)
![Immunohistochemistry-Paraffin: NFIC Antibody [NBP1-71878] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml), right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Co](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71878-img0002.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NBP2-93990-img0005.jpg "Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded rat kidney using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0004.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded human stomach using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0002.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded mouse lung using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0003.jpg)
![Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Western-Blot-NBP2-70847-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0004.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0002.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0003.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0005.jpg)
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)