| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03978SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84911-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77171-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04626-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03213-20ul | |||
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0014.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0012.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0013.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbitanti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 (lot NB100-79808-2) used for IP at 6 ug per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79808-1) and rabbit anti-RCH1/KPNA2 antibody NB100-79807. For blotting immunoprecipitated RCH1/KPNA2, NB100-79808 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79808-img0010.jpg)
![Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0002.jpg "Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: LATS1 Antibody [NB200-197] - Detection of Human LATS1 on HeLa whole cell lysate using NB200-197. LATS1 was also immunoprecipitated with another LATS1 antibody and NB200-198 using 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0001.jpg)
![Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser318]-Antibody-Western-Blot-NBP2-03978-img0003.jpg "Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.")
![Western Blot: HGS Antibody (6D11) [H00009146-M01] - HGS monoclonal antibody (M01), clone 6D11 Analysis of HGS expression in K-562.](http://images.novusbio.com/fullsize/HGS-Antibody-6D11-Western-Blot-H00009146-M01-img0010.jpg "Western Blot: HGS Antibody (6D11) [H00009146-M01] - HGS monoclonal antibody (M01), clone 6D11 Analysis of HGS expression in K-562.")
![Immunocytochemistry/Immunofluorescence: HGS Antibody (6D11) [H00009146-M01] - Analysis of monoclonal antibody to HGS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HGS-Antibody-6D11-Immunocytochemistry-Immunofluorescence-H00009146-M01-img0006.jpg)
![Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS over-expressed 293 cell line, cotransfected with HGS Validated Chimera RNAi ( Cat # H00009146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HGS monoclonal antibody (M01), clone 6D11 (Cat # H00009146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/HGS-Antibody-6D11-Western-Blot-H00009146-M01-img0008.jpg)
![Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS expression in transfected 293T cell line by HGS monoclonal antibody (M01), clone 6D11.Lane 1: HGS transfected lysate(86.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HGS-Antibody-6D11-Western-Blot-H00009146-M01-img0011.jpg)
![Sandwich ELISA: HGS Antibody (6D11) [H00009146-M01] - Detection limit for recombinant GST tagged HGS is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HGS-Antibody-6D11-Sandwich-ELISA-H00009146-M01-img0009.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0012.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunocytochemistry-Immunofluorescence-H00004782-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunohistochemistry-Paraffin-H00004782-M01-img0009.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0010.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - NFIC monoclonal antibody (M01), clone 1C12-2A2 Analysis of NFIC expression in A-431.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0011.jpg)
![Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01] - NDE1 monoclonal antibody (M01), clone 2G11-1C11 Analysis of NDE1 expression in Hela.](http://images.novusbio.com/fullsize/NDE1-Antibody-2G11-1C11-Western-Blot-H00054820-M01-img0007.jpg "Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01] - NDE1 monoclonal antibody (M01), clone 2G11-1C11 Analysis of NDE1 expression in Hela.")
![Immunocytochemistry/Immunofluorescence: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Analysis of monoclonal antibody to NDE1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NDE1-Antibody-2G11-1C11-Immunocytochemistry-Immunofluorescence-H00054820-M01-img0005.jpg)
![Sandwich ELISA: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Detection limit for recombinant GST tagged NDE1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDE1-Antibody-2G11-1C11-Sandwich-ELISA-H00054820-M01-img0006.jpg)
![Western Blot: ECRG4 Antibody [NBP1-84911] - Analysis in control (vector only transfected HEK293T lysate) and C2orf40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ECRG4-Antibody-Western-Blot-NBP1-84911-img0005.jpg "Western Blot: ECRG4 Antibody [NBP1-84911] - Analysis in control (vector only transfected HEK293T lysate) and C2orf40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Immunohistochemistry-Paraffin-NBP1-84911-img0013.jpg)
![Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Immunohistochemistry-Paraffin-NBP1-84911-img0010.jpg)
![Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Immunohistochemistry-Paraffin-NBP1-84911-img0011.jpg)
![Immunohistochemistry-Paraffin: ECRG4 Antibody [NBP1-84911] - Staining of human placenta shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Immunohistochemistry-Paraffin-NBP1-84911-img0012.jpg)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-14112025184151.jpg "<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
![<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunocytochemistry-immunofluorescence-141120251837494.jpg)
![<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-141120251842521.jpg)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-141120251841526.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-western-blot-1411202518382821.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-western-blot-1411202518324121.jpg)
![Western Blot: HGF Antibody (1D2) [NBP2-45972] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF.](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Western-Blot-NBP2-45972-img0007.jpg "Western Blot: HGF Antibody (1D2) [NBP2-45972] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF.")
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0006.jpg)
![Immunohistochemistry: HGF Antibody (OTI1D2) [NBP2-45972] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0001.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0002.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0003.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0004.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0005.jpg)
![Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Western-Blot-NBP1-77171-img0001.jpg "Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-77171] - Jurkat cells with IFITM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77171-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] - Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77171_rabbit-polyclonal-ifitm1-antibody-2711202419451297.jpg)
![Western Blot: Mkl1 Antibody [NBP3-04626] - Analysis of extracts of various cell lines, using Mkl1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/Mkl1-Antibody-Western-Blot-NBP3-04626-img0003.jpg "Western Blot: Mkl1 Antibody [NBP3-04626] - Analysis of extracts of various cell lines, using Mkl1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/ Immunofluorescence: Mkl1 Antibody - BSA Free [Mkl1] - Immunofluorescence analysis of U-2 OS cells using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04626_rabbit-polyclonal-mkl1-antibody-5220251636311.jpg)
![Immunohistochemistry: Mkl1 Antibody - BSA Free [Mkl1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-04626_rabbit-polyclonal-mkl1-antibody-52202516334167.jpg)
![Immunohistochemistry: Mkl1 Antibody - BSA Free [Mkl1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-04626_rabbit-polyclonal-mkl1-antibody-52202516321477.jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123725..jpg "IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![IHC-P-EGFR Antibody [NBP3-03213] -Human placenta using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123838..jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Mouse heart using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123952..jpg)
![Western Blot: EGFR Antibody [NBP3-03213] - analysis of A431, using EGFR Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-western-blot-611202384024..jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP3-03213] - Analysis of A-431 cells using EGFR Rabbit pAb at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunocytochemistry-immunofluorescence-611202384153..jpg)