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![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-00172-img0004.jpg "Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00172-img0001.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP1-00172-img0003.jpg)
![ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-244202310354.jpg "ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-2442023103658.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517344223.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517311071.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-5220251733559.jpg)
![Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NB100-56389-img0003.jpg "Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.")
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg "Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-26112025182908.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251843456.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251850424.jpg)
![Immunohistochemistry-Paraffin: TIMP-2 Antibody (SPM356) [NBP2-53348] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP2 Monoclonal Antibody (SPM356).](http://images.novusbio.com/fullsize/TIMP-2-Antibody-SPM356-Immunohistochemistry-Paraffin-NBP2-53348-img0001.jpg "Immunohistochemistry-Paraffin: TIMP-2 Antibody (SPM356) [NBP2-53348] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP2 Monoclonal Antibody (SPM356).")
![Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HGS-Antibody-Western-Blot-NB100-55249-img0002.jpg "Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: HGS Antibody [NB100-55249] - Whole cell lysate (15 ug) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HGS-Antibody-Western-Blot-NB100-55249-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HGS Antibody [NB100-55249] - Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody NB100-55249 (lot NB100-55249-2) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (NB100-55249-1) and another rabbit anti-HRS antibody. For blotting immunoprecipitated HRS, NB100-55249 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HGS-Antibody-Immunoprecipitation-NB100-55249-img0004.jpg)
![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0016.jpg "Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunohistochemistry-Paraffin-NBP1-06519-img0017.jpg)
![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunoprecipitation-NBP1-06519-img0014.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0008.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse liver. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0007.jpg)
![Western Blot: WT1 Antibody [NBP1-31314] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (NBP1-31314) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-31314_rabbit-polyclonal-wt1-antibody-91020242014636.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-Immunohistochemistry-Paraffin-NBP2-44496-img0003.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6)")
![Flow Cytometry: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-Flow-Cytometry-NBP2-44496-img0001.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Cervical Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-Immunohistochemistry-Paraffin-NBP2-44496-img0002.jpg)
![Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser448]-Antibody-Western-Blot-NBP2-03979-img0002.jpg "Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.")
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0007.jpg "Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.")
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in PC-12.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0006.jpg)
![Sandwich ELISA: Hexokinase 2 Antibody (4H1) [H00003099-M01] - Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Sandwich-ELISA-H00003099-M01-img0005.jpg)