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![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)
![Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Western-Blot-NBP2-50054-img0004.jpg "Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0003.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NB100-569-img0014.jpg "Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-NB100-569-img0013.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569] - Whole cell lysate (50 mcg/lane in A; 50 mcg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). NB100-569 used at 0.1 mcg/ml for Western Blot (A) and at 1 mcg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies NB100-570 and NB100-571 using 1 mcg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using NB100-570 at 0.2 mcg/ml.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NB100-569-img0009.jpg)
![Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NBP2-20782-img0004.jpg "Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.")
![Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - Expression in transfected 293T cell line by UBE2C polyclonal antibody. A: Non-transfected lysate. B: UBE2C transfected lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NBP2-20782-img0005.jpg)
![UBE2C antibody [N1C3] detects UBE2C protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UBE2C protein stained by UBE2C antibody [N1C3] (NBP2-20782) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20782_rabbit-polyclonal-ubch10-ube2c-antibody-immunocytochemistry-immunofluorescence-261120251831282.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UBE2C antibody [N1C3] (NBP2-20782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20782_rabbit-polyclonal-ubch10-ube2c-antibody-western-blot-2611202518395717.jpg)
![Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP1-28764-img0001.jpg "Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28764] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-NBP1-28764-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Septin-9 Antibody [NBP1-28764] - IP of 1mg of Hela whole cell lysate, 20% of IP loaded. Lane 1, IP using NBP1-28764. Lane 2, IP using NBP1-28711 and lane 3, IP using control IgG. Western blot probed with NBP1-28764 at 0.4ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunoprecipitation-NBP1-28764-img0002.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse brain.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0008.jpg "Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse brain.")
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in PC-12.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0004.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in IMR-32.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0005.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in human liver.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0006.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in mouse kidney.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0007.jpg)
![Western Blot: CENPH Antibody [H00064946-D01P] - Analysis of CENPH expression in transfected 293T cell line by CENPH polyclonal antibody.Lane 1: CENPH transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-D01P-img0009.jpg)
![Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Western-Blot-H00004751-M01-img0009.jpg "Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Immunohistochemistry-Paraffin-H00004751-M01-img0006.jpg)
![Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 transfected lysate using anti-NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Immunoprecipitation-H00004751-M01-img0007.jpg)
![Sandwich ELISA: NEK2 Antibody (2F6) [H00004751-M01] - Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Sandwich-ELISA-H00004751-M01-img0008.jpg)
![Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Western-Blot-H00005048-M03-img0004.jpg "Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of monoclonal antibody to PAFAH1B1 on HeLa cell. Antibody concentration 15 ug/ml](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Immunocytochemistry-Immunofluorescence-H00005048-M03-img0002.jpg)
![Sandwich ELISA: LIS1 Antibody (5A5) [H00005048-M03] - Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Sandwich-ELISA-H00005048-M03-img0003.jpg)
![ELISA: Septin-9 Antibody (2C6) [H00010801-M01] - Detection limit for recombinant GST tagged SEPT9 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Septin-9-Antibody-2C6-ELISA-H00010801-M01-img0003.jpg "ELISA: Septin-9 Antibody (2C6) [H00010801-M01] - Detection limit for recombinant GST tagged SEPT9 is 0.03 ng/ml as a capture antibody.")
![Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Western-Blot-H00000983-M01-img0011.jpg "Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Immunocytochemistry-Immunofluorescence-H00000983-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 0.3 ug/ml.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Immunohistochemistry-Paraffin-H00000983-M01-img0008.jpg)
![Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - CDC2 monoclonal antibody (M01), clone 1A4-1A9 Analysis of CDC2 expression in Hela.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Western-Blot-H00000983-M01-img0010.jpg)
![ELISA: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-ELISA-H00000983-M01-img0012.jpg)
![Western Blot: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - FZR1 monoclonal antibody (M02), clone 4C4. Analysis of FZR1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-4C4-Western-Blot-H00051343-M02-img0006.jpg "Western Blot: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - FZR1 monoclonal antibody (M02), clone 4C4. Analysis of FZR1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - Analysis of monoclonal antibody to FZR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-4C4-Immunohistochemistry-Paraffin-H00051343-M02-img0004.jpg)
![Western Blot: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - Western Blot analysis of FZR1 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-4C4-Western-Blot-H00051343-M02-img0001.jpg)
![Proximity Ligation Assay: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - Analysis of protein-protein interactions between CCNB1 and FZR1. HeLa cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-FZR1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-4C4-Proximity-Ligation-Assay-H00051343-M02-img0002.jpg)
![Sandwich ELISA: FZR1/CDH1 Antibody (4C4) [H00051343-M02] - Detection limit for recombinant GST tagged FZR1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-4C4-Sandwich-ELISA-H00051343-M02-img0005.jpg)