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![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-2120249393515.jpg "Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939177.jpg)
![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939148.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024941456.jpg)
![Immunoprecipitation of Rad21 protein from HeLa whole cell extract using 5 ug of Rad21 antibody [HL2171] (NBP3-25619).Western blot analysis was performed using Rad21 antibody [HL2171] (NBP3-25619).EasyBlot HRP-conjugated anti rabbit IgG antibody .](http://images.novusbio.com/fullsize/nbp1-32470_rabbit-polyclonal-p2x7-p2rx7-antibody-immunohistochemistry-frozen-141120251855419.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25722_rabbit-thymidylate-synthase-mab-hl1237-azide-bsa-free-212024939352.jpg "Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25722_rabbit-thymidylate-synthase-mab-hl1237-azide-bsa-free-21202494140.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25722_rabbit-thymidylate-synthase-mab-hl1237-azide-bsa-free-2120249383147.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Thymidylate synthase antibody [HL1237] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. Thymidylate synthase stained by Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25722_rabbit-thymidylate-synthase-mab-hl1237-azide-bsa-free-212024939138.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0005.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0001.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP1-76298-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody - BSA Free [NBP1-76298] - Immunofluorescence of Importin alpha 2/KPNA2 in human heart tissue with Importin alpha 2/KPNA2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76298_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-2711202419432251.jpg)
![Western Blot: CENPH Antibody (2G4) [NBP2-01449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH.](http://images.novusbio.com/fullsize/CENPH-Antibody-2G4-Western-Blot-NBP2-01449-img0002.jpg "Western Blot: CENPH Antibody (2G4) [NBP2-01449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH.")
![Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] Staining of paraffin-embedded Human tonsil using anti-CENPH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CENPH-Antibody-2G4-Immunohistochemistry-Paraffin-NBP2-01449-img0005.jpg)
![Flow Cytometry: CENPH Antibody (2G4) [NBP2-01449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CENPH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CENPH-Antibody-2G4-Flow-Cytometry-NBP2-01449-img0001.jpg)
![Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CENPH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CENPH-Antibody-2G4-Immunohistochemistry-Paraffin-NBP2-01449-img0003.jpg)
![Immunohistochemistry-Paraffin: CENPH Antibody (2G4) [NBP2-01449] - Staining of paraffin-embedded Human lung tissue using anti-CENPH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CENPH-Antibody-2G4-Immunohistochemistry-Paraffin-NBP2-01449-img0004.jpg)
![Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-06076-img0002.jpg "Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0003.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Tonsil tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0004.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0004.jpg "Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: Septin-11 Antibody [NBP1-58144] - Human Spleen Tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Immunohistochemistry-Paraffin-NBP1-58144-img0003.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody : IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0001.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - NT-2 cell line, mouse brain, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0002.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0005.jpg)
![Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NB100-1100-img0003.jpg "Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0002.jpg)
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0001.jpg)
![Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Western-Blot-NBP3-15627-img0009.jpg "Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Knockout-Validated-NBP3-15627-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100 x.](http://images.novusbio.com/fullsize/nbp3-15627_rabbit-cyclin-d1-mab-7c8z4-immunocytochemistry-immunofluorescence-2092023105125.jpg)
![Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Western-Blot-NBP3-15798-img0004.jpg "Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunoprecipitation-NBP3-15798-img0005.jpg)
![Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Western-Blot-NBP3-16529-img0003.jpg "Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded human colon using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded rat testis using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0001.jpg)
![Immunoprecipitation: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug MCM5 antibody (NBP3-16529). Western blot was performed from the immunoprecipitate using MCM5 antibody (NBP3-16529) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunoprecipitation-NBP3-16529-img0004.jpg)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)